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Production of human recombinant neurotrophins for biomedical studies

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Abstract

The genes of nerve growth factor (NGF), brain neurotrophic factor (BDNF) and human neurotrophic factor 3 (NT-3) were cloned and expressed in Escherichia coli. Methods of purification and renaturation of the proneurotrophins were developed. It was shown that the recombinant pro-NGF, pro-BDNF and pro-NT-3 induce a differentiation of chicken dorsal root ganglia cell culture.

About the Authors

L. M. Rafieva
Институт молекулярной генетики РАН
Russian Federation


D. R. Safina
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


I. V. Demidyuk
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


S. V. Kostrov
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


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For citation:


Rafieva L.M., Safina D.R., Demidyuk I.V., Kostrov S.V. Production of human recombinant neurotrophins for biomedical studies. Fine Chemical Technologies. 2011;6(2):51-57. (In Russ.)

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ISSN 2686-7575 (Online)