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Production of human recombinant neurotrophins for biomedical studies

Abstract

The genes of nerve growth factor (NGF), brain neurotrophic factor (BDNF) and human neurotrophic factor 3 (NT-3) were cloned and expressed in Escherichia coli. Methods of purification and renaturation of the proneurotrophins were developed. It was shown that the recombinant pro-NGF, pro-BDNF and pro-NT-3 induce a differentiation of chicken dorsal root ganglia cell culture.

About the Authors

L. M. Rafieva
Институт молекулярной генетики РАН
Russian Federation


D. R. Safina
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


I. V. Demidyuk
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


S. V. Kostrov
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


References

1. Segal R.A. Selectivity in neurotrophin signaling: Theme and variations // Annu. Rev. Neurosci. 2003. V. 26. P. 299-330.

2. Pedraza S.E., Podlesniy P., Vidal N., Arévalo J.C., Lee R., Hempstead B., Ferrer I., Iglesias M., Espinet C. Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR / // Am. J. Pathol. 2005. V. 166. № 2. P. 533-543.

3. Nordell V.L., Lewis D.K., Bake S., Sohrabji F. The neurotrophin receptor p75NTR mediates early anti-inflammatory effects of estrogen in the forebrain of young adult rats // BMC Neurosci. 2005. V. 6. P. 58. URL: <http://www.biomedcentral.com/1471-2202/6/58>

4. McDonald N.Q., Chao M.V. Structural determinants of neurotrophin action // J. Biol.Chem. 1995. V. 270. P. 19669-19672.

5. Reichardt L.F. Neurotrophin-regulated signalling pathways // Philos. Trans. R. Soc. Lond. B. Biol. Sci. 2006. V. 361. № 1473. P. 1545-1564.

6. Arévalo J.C., Wu S.H. Neurotrophin signaling: Many exciting surprises! // Cell Mol. Life Sci. 2006. V. 63. № 13. P. 1523-1537.

7. Jones K.R. Reichardt L.F. Molecular cloning of human gene that is member of nerve growth factor family // Proc. Natl. Acad. Sci. USA. 1990. V. 87. № 20. P. 8060-8064.

8. McDonald, N.Q., Hendrickson W.A. A structural superfamily of growth factors containing a cystine knot motif // Cell. 1993. V. 73. № 3. P. 421-424.

9. Lessmann V., Gottmann K., Malcangio M. Neurotrophin secretion: current facts and future prospects // Prog. Neurobiol. 2003. Vol. 69. № 5. P. 341-374.

10. Rattenholl A., Lilie H., Grossmann A., Stern A., Schwarz E., Rudolph R. The pro-sequence facilitates folding of human nerve growth factor from Escherichia coli inclusion bodies // Eur. J. Biochem. 2001. V. 268. № 11. P. 3296-3303.

11. Rattenholl A., Ruoppolo M., Flagiello A., Monti M., Vinci F., Marino G., Lilie H., Schwarz E., Rudolph R. Pro-sequence assisted folding and disulfide bond formation of human nerve growth factor // J. Mol. Biol. 2001. V. 305. № 3. P. 523-533.

12. Schweigreiter R. The dual nature of neurotrophins // Bioessays. 2006. V. 28. № 6. P. 583-594.

13. Paoletti F., Konarev P.V., Covaceuszach S., Schwarz E., Cattaneo A., Lamba D., Svergun D.I. Structural and functional properties of mouse proNGF // Biochem. Soc. Trans. 2006. V. 34. № 4. P. 605-606.

14. Fahnestock M., Michalski B., Xu B., Coughlin M.D. The precursor pro-nerve growth factor is the predominant form of nerve growth factor in brain and is increased in Alzheimer's disease // Mol. Cell. Neurosci. 2001. V. 18. № 2. P. 210-220.

15. Lee R., Kermani P., Teng K.K., Hempstead B.L. Regulation of cell survival by secreted proneurotrophins // Science. 2001. V. 294. № 5548. P. 1945-1948.

16. Teng H.K., Teng K.K., Lee R. [et al.]. ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin // J. Neurosci. 2005. Vol. 25. № 22. P. 5455-5463.

17. Pagadala P.C., Dvorak L.A., Neet K.E. Construction of a mutated pro-nerve growth factor, resistant to degradation and suitable for biophysical and cellular utilization // Proc. Natl. Acad. Sci. USA. 2006. V. 103. № 47. P. 17939-17943.

18. Kliemannel M., Rattenholl A., Golbik R., Balbach J., Lilie H., Rudolph R., Schwarz E. The mature part of proNGF induces the structure of its pro-peptide // FEBS Lett. 2004. V. 566. № 1-3. P. 207-212.

19. Volosin M., Trotter C., Cragnolini A., Kenchappa R.S., Light M., Hempstead B.L., Carter B.D., Friedman W.J. Induction of proneurotrophins and activation of p75NTR-mediated apoptosis via Neurotrophin Receptor Interacting Factor (NRIF) in hippocampal neurons after seizures / J. Neurosci. 2008. V. 28. № 39. P. 9870-9879.

20. Edwards R.H., Selby M.J., Garcia P.D., Rutter W.J. Processing of the native nerve growth factor precursor to form biologically active nerve growth factor // J. Biol. Chem. 1988. V. 263. № 14. P. 6810-6815.

21. Yano H., Torkin R., Martin L.A., Chao M.V., Teng K.K. Proneurotrophin-3 is a neuronal apoptotic ligand: Evidence for retrograde-directed cell killing // J. Neurosci. 2009. V. 29. № 47. P. 14790-14802.

22. Koshimizu H., Kiyosue K., Hara T. [et al.]. Multiple functions of precursor BDNF to CNS neurons: negative regulation of neurite growth, spine formation and cell survival // Mol. Brain. 2009. V. 2. P. 27. URL: <http://www.molecularbrain.com/content/2/1/27>

23. Zhang D., Bernd P. Bernd expression of TRK and neurotrophin mRNA in dorsal root and sympathetic of the quail during development // J. Neurobiol. 1994. V. 25. № 12. P. 1517-1532.

24. Young L., Bisland J., Harper S. A rapid method for determination of cell survival in primary neuronal DRG cultures // J. Neurosci. Methods. 1999. V. 93. № 1. P. 81-89.

25. Greene L.A. Quantitative in vitro studies on the nerve growth factor (NGF) requirement of neurons. II. Sensory neurons // Dev. Biol. 1977. V. 58. № 1. P. 106-113.

26. Rosenthal A., Goeddel D., Winslow W. Primary structure and biological activity of a novel human neurotropthic factor // Neuron. 1990. V. 4. № 5. P. 767-773.

27. Laemmli U.K. Cleavage of structural proteins during the assembly of the head bacteriophage T4 // Nature. 1970. V. 227. № 5259. P. 680-685.

28. McCarthy K., Partlow L. Preparation of pure neuronal and non-neuronal cultures from embryonic chick sympathetic ganglia a new method based on both differential cell adhesiveness and the formation of homotypic neuronal aggregates // Brain Res. 1976. V. 114. № 3. P. 391-414.

29. Davies A. Neurotrophic factor bioassay using dissociated neurons // In: Nerve growth factors / Ed. R.A. Rush. - New York: John Wiley and Sons, 1989. Chapt. 5. P. 95-109.


Review

For citations:


Rafieva L.M., Safina D.R., Demidyuk I.V., Kostrov S.V. Production of human recombinant neurotrophins for biomedical studies. Fine Chemical Technologies. 2011;6(2):51-57. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)