Preview

Fine Chemical Technologies

Advanced search

A New Technology for Extraction of Essential Oil from Anethum graveolens L. Fruits

https://doi.org/10.32362/2410-6593-2019-14-2-33-40

Abstract

The aim of this work was to study the possibility of perfluoroorganic solvents use for extraction of main compounds of essential oil from Anethum graveolens L. fruits. We used Anethum graveolens L. fruits with particle size of 0.1–0.5 mm for extraction. We used the method of simple maceration for 24 hours, with raw material to solvent ratio of 1:10 w/v at 24±1°С. Besides, we used the method of circulation extraction in the Soxhlet extractor with raw material to solvent ratio of 1:5 w/v for 1 to 4 hours. The qualitative analysis of extracts was carried out by the method of GC-MS. The quantitative analysis of carvone was carried out by the method of RP HPLC at 240 nm analytical wavelength. The use of simple maceration method provided the following yields of carvone into all types of solvents: 95±4 % for n-hexane, 95±4 % for Novec 7100, and 69±3 % for Novec 1230. The use of the circulation method of extraction for 60 min of solvent circulation provided the following yields of carvone: 93±4 % for Novec 1230 and 92±4 % for Novec 7100. It was noted that the extract obtained with solvent Novec 7100 was contaminated by fatty oil and required an addition stage of purification. The results obtained suggest that perfluoro organic solvents have good prospects and applicability in the phytotechnology for the extraction of some types of biologically active compounds and essential oils from the plant raw material.

About the Authors

N. N. Boyko
Belgorod National Research University
Russian Federation

h.D. (Pharmacy), Junior Research Scientist of the Laboratory of Technology of Drugs, Associate Professor of the Chair of Pharmaceutical Technology

85, Pobedy st., Belgorod, 308015, Russia



D. I. Pisarev
Belgorod National Research University
Russian Federation

D.Sc. (Pharmacy), Professor of the Chair of General Chemistry

85, Pobedy st., Belgorod, 308015, Russia



E. T. Zhilyakova
Belgorod National Research University
Russian Federation

D.Sc. (Pharmacy), Professor, Head of the Chair of Pharmaceutical Technology

85, Pobedy st., Belgorod, 308015, Russia



A. Yu. Malyutina
Belgorod National Research University
Russian Federation

Ph.D. (Pharmacy), Associate Professor of the Chair of General Chemistry

85, Pobedy st., Belgorod, 308015, Russia



O. O. Novikov
Test Department "Drugs Quality Control Center" of the Shared Research and Education Center, Peoples’ Friendship University of Russia
Russian Federation

D.Sc. (Pharmacy), Professor, Head

8/2, Miklukho-Maklay st., Moscow, 117198, Russia



References

1. Popova N.V., Litvinenko V.I., Kutsanyan A.S. Medicinal Plants of the World: Encyclopedic Handbook. Kharkov: Disa Plus Publ., 2016. 540 p. (in Russ.)

2. Al-Snafi E.A. The pharmacological importance of Anethum graveolens. A review. Int. J. Pharmacy and Pharm. Sci. 2014; 6(4):11-13.

3. Yousofvand N., Soltany A. Effects of hydroalcoholic extract of dill (Anethum graveolens) on the serum levels of blood lipids (cholesterol, triglycerides, LDL and HDL) in male NMRI mice J. Pharm., Chem. and Biol. Sci. 2015; 3(1):114-121.

4. Chueshov V.I., Gladukh E.V., Sayko I.V. [et al.] Technology of manufactured drugs: transl. from Ukrainian. Vinniysa: Nonavaya Kniga Publ., 2014. V. 1. 696 p. (in Russ.)

5. Kas’yanov G.I., Pekhov A.V., Taran A.A. Natural food flavors – CO2 extracts. Moscow: Pishchevaya promyshlennost’ (Food Industry) Publ., 1978. 176 p. (in Russ.)

6. Technology and standardization of drugs: Collection of research papers. Ed. by V.P. Georgievskiy and F.A. Konev. Kharkov: LLC “RIREG” Publ., 2000. 784 p. (in Russ.)

7. Zilfikarov I.N., Chelombit’ko V.A., Aliev A.M. Treatment of the plant raw material by liquefied gases and supercritical fluids. Pyatigorsk, 2007. 244 p. (in Russ.)

8. Industrial Solvents Handbook. Ed. by Ernest W. Flick. 5th ed. Westwood, New Jersey: Noyes Data Corporation Publ., 1998. 994 p.

9. Lemaire B., Mompon B., Surbled I., Surbled M. Method for fractionating essential oils using at least fluorinated solvent: Pat. EP 1307533B1. Priority Date 06/22/2000.

10. Lemaire B., Surbled M., Zwegers J., Mompon B. Method for extracting and fractionating fats with solvent, using at least a hydrofluroether: Pat. US 6673952 B2. Pub. Date: 01/06/2004.

11. New in the technology of fluorinated compounds: transl. from Japanese. Ed. by N.I. Isikava. Moscow: Mir Publ., 1984. 592 p. (in Russ.)

12. State pharmacopoeia of Russian Federation. XIV ed. Vol. I. Moscow: Ministry of Healthcare of Russian Federation Publ., 2018. Access: http://femb.ru/femb/pharmacopea.php. Access Date 03/06/19.

13. Zhilyakova E.T., Novikov O.O., Pisarev D.I., Malyutina A.Y., Boyko N.N. Studying the polyphenolic structure of Laurus nobilis L. leaves. Indo Am. J. Pharm. Sci. 2017; 4(9): 3066-3074.

14. Zorca M., Gainar I., Bala D. The CO2 density variation in the supercritical extraction of anet essential oils. Analele Universităńii din Bucuresti – Chimie, Anul. – XVI (serie nouă). 2007; I:43-47.

15. Nenov N., Atanasova T., Stoilova I., Gochev V., Girova T., Stoyanova A. Low temperature extraction of essential oil bearing plants by liquificate gases. 11. Dill (Anethum graveolens L.). Scientific works. Vol. LX. Food Science, Engineering and Technologies. 18-19 October 2013. Plovdiv, 2013.


Supplementary files

1. Fig. 3. The dependence of the yield of carvone from the circulation time of the solvent Novec 1230/7100. Repeat count n = 3, significance level Р = 0.95.
Subject
Type Research Instrument
View (61KB)    
Indexing metadata

Review

For citations:


Boyko N.N., Pisarev D.I., Zhilyakova E.T., Malyutina A.Yu., Novikov O.O. A New Technology for Extraction of Essential Oil from Anethum graveolens L. Fruits. Fine Chemical Technologies. 2019;14(2):33-40. (In Russ.) https://doi.org/10.32362/2410-6593-2019-14-2-33-40

Views: 7854


ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)