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DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL

https://doi.org/10.32362/2410-6593-2017-12-2-81-99

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Abstract

In the present study, a rapid, sensitive, and selective method for determination of several synthetic analogues of gonadotropin-releasing hormone in human urine by solid-phase extraction and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed. Various parameters affecting sample preparation, LC separation, and MS/MS detection were investigated, and optimized conditions were identified. The UPLC-MS/MS system was equipped with an electrospray ion source operating in positive ion mode with selected reaction monitoring. Leuprolide-13С6 was used as internal standard for analytes quantitative assessment. The proposed method was validated considering the parameters specificity, linearity (0.1-10 ng/ml), recovery (52-98%), limit of detection (0.1 ng/ml), matrix effects and stability.

About the Authors

K. M. Shestakova
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


G. A. Dudko
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


M. A. Dikunets
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


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For citation:


Shestakova K.M., Dudko G.A., Dikunets M.A. DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL. Fine Chemical Technologies. 2017;12(2):81-99. (In Russ.) https://doi.org/10.32362/2410-6593-2017-12-2-81-99

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)