Комбинация Phyllanthus amarus Schum. & Thonn. и Gymnema sylvestre R. Br. для лечения диабета и его долгосрочных осложнений

Цели. Количество пациентов с диабетом растет, у них часто возникают долгосрочные осложнения, поэтому поиск методов лечения этого заболевания и коррекции его осложнений является важным для всего мирового медицинского сообщества. Phyllanthus amarus Schum. & Thonn. (PA) и Gymnema sylvestre R. Br. (GS) – распространенные во Вьетнаме лекарственные растения, используемые в традиционной медицине, включая лечение диабета. Цель данного исследования – скомбинировать PA и GS, чтобы расширить их биологическую активность и усилить антидиабетический, антиоксидантный и противовоспалительный эффект.
Методы. Порошки листьев PA и GS смешивали в различных соотношениях и экстрагировали 95% этанолом. Полученные этанольные экстракты использовались для определения биологически активных соединений, биологической активности и оптимального соотношения компонентов смеси.
Результаты. Оптимальное соотношение PA и GS, определенное в исследовании, равно 2 : 1 (г/г). Экстракция 95% этанолом данного образца (2 : 1) при 50 °C в течение двух часов при соотношении сырье/эстрагент 1 : 10 позволила получить высокий выход экстрактивных веществ, равный 14.37%. Этот образец продемонстрировал хорошую активность ингибирования α-глюкозидазы с половинной максимальной ингибирующей концентрацией (IC50) 9.74 мкг/мл, антиоксидантную активность с IC50 29.87 мкг/мл и противовоспалительную активность с IC15 400 мкг/мл.
Выводы. Исследование подтвердило, что сочетание PA и GS может значительно ингибировать α-глюкозидазу, а также обладает антиоксидантным и противовоспалительным эффектами.

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