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Combination of Phyllanthus amarus Schum. & Thonn. and Gymnema sylvestre R. Br. for treatment of diabetes and its long-term complications

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Objectives. The amount of patients with diabetes is increasing, and such patients experience several long-term complications. Therefore, finding a method to treat the disease and its complications is an urgent issue worldwide. In Vietnam, Phyllanthus amarus Schum. & Thonn. (PA) and Gymnema sylvestre R. Br. (GS) are common herbs used in traditional therapy including diabetes treatment. This study aimed to combine PA and GS to extend their bioactivities in antidiabetes, antioxidant, and anti-inflammatory treatments.
Methods. Here, PA and GS powders were mixed at different ratios for extraction. Ethanolic extract was used to detect bioactive compounds, bioactivities, and appropriate ratios of the mixtures.
Results. The optimal ratio for the PA and GS combination was 2 : 1 (g/g). The ethanolic extraction of the 2 : 1 sample at 50°C over two hours with a solid/liquid ratio of 1 : 10 achieved a high yield of 14.37%. This sample exhibited good α-glucosidase inhibition activity with a half-maximal inhibitory concentration (IC50) of 9.74 µg/mL, antioxidant activity with an IC50 of 29.87 µg/mL, and anti-inflammatory activity with an IC15 of 400 µg/mL.
Conclusions. The study confirmed that combining PA and GS can have high α-glucosidase inhibition as well as antioxidant and anti-inflammatory effects.

About the Authors

T. M. Le
Faculty of Chemical Engineering, Ho Chi Minh City University of Technology; Vietnam National University Ho Chi Minh City
Viet Nam

Tan M. Le, BEng., Postgraduate Student

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 

Ch. D.P. Nguyen
Faculty of Chemical Engineering, Ho Chi Minh City University of Technology; Vietnam National University Ho Chi Minh City
Viet Nam

Chinh D.P. Nguyen, BEng., Postgraduate Student

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 

A. C. Ha
Faculty of Chemical Engineering, Ho Chi Minh City University of Technology; Vietnam National University Ho Chi Minh City
Viet Nam

Anh C. Ha,PhD, Dr. of Chemical Engineering

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 


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Supplementary files

1. Comparison of the samples’ bioactivities, extraction yields, and TPE
Type Исследовательские инструменты
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2. This is to certify that the paper titled Combination of Phyllanthus amarus Schum. & Thonn. and Gymnema sylvestre R. Br. for treatment of diabetes and its long-term complications commissioned to us by Tan M. Le, Chinh D.P. Nguyen, Anh C. Ha has been edited for English language and spelling by Enago, an editing brand of Crimson Interactive Inc.
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Indexing metadata
  • For the first time, Phyllanthus amarus Schum. & Thonn. (PA) and Gymnema sylvestre R. Br. (GS) was combined for treatment of antidiabetes.
  • The combination of PA and GS achieved high bioactivities, such as antidiabetes, antioxidant, and anti-inflammatory.
  • The mixture with the PA/GS ratio of 2 : 1 was the best sample because of its high total polyphenol excreted and antidiabetes and related bioactivities.


For citations:

Le T.M., Nguyen C.D., Ha A.C. Combination of Phyllanthus amarus Schum. & Thonn. and Gymnema sylvestre R. Br. for treatment of diabetes and its long-term complications. Fine Chemical Technologies. 2021;16(3):232-240.

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