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Flavonoid-profile determination for a hypoglycemic collection by high-performance liquid chromatography

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Objectives. Herbal hypoglycemic drugs complement the conventional approach to the treatment of type-2 diabetes based on the use of synthetic prescription drugs. However, their scientifically based application and standardization are limited due to inadequate and often outdated information on their chemical composition. Accordingly, we have developed a hypoglycemic collection (HGС) consisting of common bean pods (Phaseolus vulgaris L.), bilberry shoots (Vaccinium myrtillus L.), galega herb (Gallega officinalis L.), common knotgrass herb (Polygonum aviculare L.), burdock roots (Arctium lappa L.), and cinnamon rose hips (Rosa cinnamomea L.). According to a number of researchers, the antidiabetic properties of these herbs are largely due to the presence of polyphenolic compounds, especially flavonoids. The aim of this study was to determine the profile of flavonoids in the HGС and in its total dry extract (TDE).

Methods. The study was performed by reverse-phase high-performance liquid chromatography with diode array and mass spectrometric detection.

Results. Nine individual flavonol glycosides—derivatives of myricetin, quercetin, kaempferol and kaempferide—were identified in the HGC and the TDE. The main flavonol glycosides in the studied objects were robinin and kaempferol-3-glucuronide, the contents of which in the HGC were 2.09 and 2.22 mg/g, in the TDE 4.85 and 3.84 mg/g, respectively. The other flavonol glycosides were determined in the HGC and its TDE at significantly lower concentrations.

Conclusions. The method developed in the study can be used to standardize HGCs and estimate their pharmacological activities.

About the Authors

A. M. Kutovaya
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR)
Russian Federation

Angelina M. Kutovaya, Postgraduate Student. ResearcherID AAK-6219-2020

7, ul. Grina, Moscow, 117216

V. N. Davydova
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR)
Russian Federation

Valentina N. Davydova, Dr. of Sci. (Pharm.), Chief Researcher, Pharmaceutical Technology Department. Scopus Author ID 57195220369

7, ul. Grina, Moscow, 117216

I. B. Perova
Federal Research Center of Nutrition, Biotechnology and Food Safety
Russian Federation

Irina B. Perova, Cand. of Sci. (Pharm.), Senior Researcher, Laboratory of Metabolomic and Proteomic Analysis. ResearcherID K-6257-2018, Scopus Author ID 56403250700

2/14, Ust’inskii proezd, Moscow, 109240

K. I. Eller
Federal Research Center of Nutrition, Biotechnology and Food Safety
Russian Federation

Konstantin I. Eller, Dr. of Sci. (Chemistry), Professor, Head of the Laboratory of Metabolomic and Proteomic Analysis. Researcher ID K-6408-2018, Scopus Author ID 7003969952

2/14, Ust’inskii proezd, Moscow, 109240


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Supplementary files

1. Chromatogram of the hypoglycemic collection extract at λ = 350 nm. The peak numbers correspond to the flavonoid numbers in Tables 1 and 2.
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2. This is to certify that the paper titled Flavonoid-profile determination for a hypoglycemic collection by high-performance liquid chromatography commissioned to Enago by Angelina M. Kutovaya, Valentina N. Davydova, Irina B. Perova, Konstantin I. Eller has been edited by Enago, an editing brand of Crimson Interactive Inc.
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The flavonoid profile of hypoglycemic collection (HGC) consisting of the leaves of the common bean (Phaseolus vulgaris L.), shoots of the common blueberry (Vaccinium myrtillus L.), herb of galega (Galega officinalis L.), grass of the mountaineer (knotweed) (Polygonum aviculare L.), roots of burdock (Arctium lappa L.), and cinnamon rose hips (Rosa cinnamomea L.) was initially investigated by reversed-phase high-performance liquid chromatography utilizing an online diode array detector and a mass spectrometer. Nine individual flavonol glycosides, derivatives of myricetin, quercetin, kaempferol, and kaempferid, were identified in HGC. The total content of flavonoids in HGC and in dry extract was determined at 6.46 and 13.96 mg/g, respectively. Robinin and kaempferol-3-glucuronide were identified as the main flavonol glycosides, the content of which in HGC was 2.09 and 2.22 mg/g, respectively.

For citation:

Kutovaya A.M., Davydova V.N., Perova I.B., Eller K.I. Flavonoid-profile determination for a hypoglycemic collection by high-performance liquid chromatography. Fine Chemical Technologies. 2020;15(3):39-46.

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