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APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL

https://doi.org/10.32362/2410-6593-2017-12-1-64-75

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Abstract

The article presents the results of the first stage of development of a new quantitative method for human chorionic gonadotropin (hCG) determination by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) to uncover doping abuse by athletes. The identified tryptic peptides correspond to the most abundant hCG isoforms: the α- and β-subunits, the nicked and β-core fragment of the hormone. Identification and sequencing of specific fragments were performed with the use of nanoLC-MS/MS. A high resolution / high accuracy hybrid mass-spectrometer was applied. Optimization of mass-spectrometric determination of selected specific peptides was accomplished by UPLC-MS/MS. Quantitative evaluation of hCG using specific fragments determination by UPLC-MS/MS allows to detect corresponding hCG isoforms. This significantly increases the method specificity and decreases the probability of false-positive results.

About the Authors

I. O. Zvereva
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


N. B. Savelieva
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


P. V. Postnikov
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


Yu. A. Efimova
Moscow Technological University (Institute of Fine Chemical Technologies)
Russian Federation
Moscow, 119571 Russia


M. A. Dikunets
Federal State Budgetary Institution “Antidoping Centre”
Russian Federation
Moscow, 105005 Russia


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For citation:


Zvereva I.O., Savelieva N.B., Postnikov P.V., Efimova Yu.A., Dikunets M.A. APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL. Fine Chemical Technologies. 2017;12(1):64-75. (In Russ.) https://doi.org/10.32362/2410-6593-2017-12-1-64-75

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)