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Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

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Abstract

A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.

Keywords


57.088.3:543.544.52, 577.113.6, 577.113.083

About the Authors

A. G. Mosina
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


A. N. Chuvilin
Научно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства России
Russian Federation


I. P. Smirnov
Научно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства России
Russian Federation


V. F. Travkin
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


G. E. Pozmogova
МИТХТ им. М.В. Ломоносова
Russian Federation


References

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For citation:


Mosina A.G., Chuvilin A.N., Smirnov I.P., Travkin V.F., Pozmogova G.E. Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking. Fine Chemical Technologies. 2012;7(4):72-74. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)