Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
Abstract
A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.
Keywords
57.088.3:543.544.52, 577.113.6, 577.113.083
About the Authors
A. G. Mosina
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Russian Federation
A. N. Chuvilin
Научно-исследовательский институт физико-химической медицины
Федерального медико-биологического агентства России
Russian Federation
Russian Federation
I. P. Smirnov
Научно-исследовательский институт физико-химической медицины
Федерального медико-биологического агентства России
Russian Federation
Russian Federation
V. F. Travkin
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Russian Federation
G. E. Pozmogova
МИТХТ им. М.В. Ломоносова
Russian Federation
Russian Federation
References
1. Yeung A.T., Holloway B.P., Adams P.S. Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR // Biotechniques. 2004. V. 36. P. 266-270.
2. Wilkening S., Bader A. Quantitative real-time polymerase chain reaction: Methodical analysis and mathematical model // J. Biomol. Tech. 2004. V. 15. P. 107-111.
3. Juskowiak B. Nucleic acid-based fluorescent probes and their analytical potential // Anal. Bioanal. Chem. 2011. V. 399. P. 3157-3176.
4. Marras S.A., Tyagi S., Kramer F.R. Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes // Clin. Chim. Acta. 2006. V. 363. P. 48-60.
5. Chuvilin A.N., Serebryakova M.V., Smirnov I.P., Pozmogova G.E. Byproduct with altered fluorescent properties is formed during standard deprotection step of hexachlorofluorescein labeled oligonucleotides // Bioconjugate Chem. 2009. V. 20. P 1441-1443.
Review
For citations:
Mosina A.G., Chuvilin A.N., Smirnov I.P., Travkin V.F., Pozmogova G.E. Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking. Fine Chemical Technologies. 2012;7(4):72-74. (In Russ.)
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