chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).chemicallytech-719Research ArticleCHEMISTRY AND TECHNOLOGY OF MEDICINAL COMPOUNDS AND BIOLOGICALLY ACTIVE SUBSTANCESХИМИЯ И ТЕХНОЛОГИЯ ЛЕКАРСТВЕННЫХ ПРЕПАРАТОВ И БИОЛОГИЧЕСКИ АКТИВНЫХ СОЕДИНЕНИЙAnalysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblockingАнализ эффективности очистки HEX-олигонуклеотидов, полученных с использованием модифицированного метода деблокированияМосинаА. Г.MosinaA. G.AlenaGMosina@yandex.ruЧувилинА. Н.ChuvilinA. N.noemail@neicon.ruСмирновИ. П.SmirnovI. P.noemail@neicon.ruТравкинВ. Ф.TravkinV. F.noemail@neicon.ruПозмоговаГ. Е.PozmogovaG. E.noemail@neicon.ruМИТХТ им. М.В. ЛомоносоваРоссияM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Russian FederationНаучно-исследовательский институт физико-химической медицины
Федерального медико-биологического агентства РоссииRussian FederationНаучно-исследовательский институт физико-химической медицины
Федерального медико-биологического агентства РоссииРоссияM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Russian FederationМИТХТ им. М.В. ЛомоносоваRussian Federation201228082012747274Copyright © Mosina A.G., Chuvilin A.N., Smirnov I.P., Travkin V.F., Pozmogova G.E., 20122012Мосина А.Г., Чувилин А.Н., Смирнов И.П., Травкин В.Ф., Позмогова Г.Е.Mosina A.G., Chuvilin A.N., Smirnov I.P., Travkin V.F., Pozmogova G.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/719гексахлорфлуоресцеинолигодезоксирибонуклеотидыВЭЖХПЦР в режиме ре-ального времениhexachlorofluorescein lаbeloligodeoxyribonucleotidesHPLCreal time PCRReferencesYeung A.T., Holloway B.P., Adams P.S. Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR // Biotechniques. 2004. V. 36. P. 266-270.Yeung A.T., Holloway B.P., Adams P.S. Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR // Biotechniques. 2004. V. 36. P. 266-270.Wilkening S., Bader A. Quantitative real-time polymerase chain reaction: Methodical analysis and mathematical model // J. Biomol. Tech. 2004. V. 15. P. 107-111.Wilkening S., Bader A. Quantitative real-time polymerase chain reaction: Methodical analysis and mathematical model // J. Biomol. Tech. 2004. V. 15. P. 107-111.Juskowiak B. Nucleic acid-based fluorescent probes and their analytical potential // Anal. Bioanal. Chem. 2011. V. 399. P. 3157-3176.Juskowiak B. Nucleic acid-based fluorescent probes and their analytical potential // Anal. Bioanal. Chem. 2011. V. 399. P. 3157-3176.Marras S.A., Tyagi S., Kramer F.R. Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes // Clin. Chim. Acta. 2006. V. 363. P. 48-60.Marras S.A., Tyagi S., Kramer F.R. Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes // Clin. Chim. Acta. 2006. V. 363. P. 48-60.Chuvilin A.N., Serebryakova M.V., Smirnov I.P., Pozmogova G.E. Byproduct with altered fluorescent properties is formed during standard deprotection step of hexachlorofluorescein labeled oligonucleotides // Bioconjugate Chem. 2009. V. 20. P 1441-1443.Chuvilin A.N., Serebryakova M.V., Smirnov I.P., Pozmogova G.E. Byproduct with altered fluorescent properties is formed during standard deprotection step of hexachlorofluorescein labeled oligonucleotides // Bioconjugate Chem. 2009. V. 20. P 1441-1443.
The authors declare that there are no conflicts of interest present.