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Technology for test-systems for diagnosis of modified proteins (proteopathy) underlying social diseases (Report 1)

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Abstract

A new experimental technique for obtaining diagnostic test systems is introduced based on conjugates "polymer microsphere–bioligand" for rapid diagnosis of autoantibodies to the modified protein on example of thyroglobulin, which consists of preliminary blocking of aldehyde groups of polymer microspheres by glycine amine groups, and then of the covalent interaction between carboxyl group of glycine and the amino groups of thyroglobulin. This way of creating test systems has enhanced the sensitivity of the reaction of latex agglutination (RLA) by extending the boundaries of titer.

About the Authors

Ya. M. Stanishevskiy
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Department of Biomedical and Pharmaceutical Technology,  associate professor


I. A. Gritskova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Department of Chemical Technology of Macromolecular Compounds, professor


N. S. Kuzmina
Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064
Russian Federation
Laboratory of Immunological Diagnosis of Endocrine Diseases, head of the laboratory


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For citation:


Stanishevskiy Y.M., Gritskova I.A., Kuzmina N.S. Technology for test-systems for diagnosis of modified proteins (proteopathy) underlying social diseases (Report 1). Fine Chemical Technologies. 2013;8(3):87-94. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)