chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).chemicallytech-597Research ArticleTO THE 150TH ANNIVERSARY OF THE BIRTH OF V. I. VERNADSKYTechnology for test-systems for diagnosis of modified proteins (proteopathy) underlying social diseases (Report 1)Technology for test-systems for diagnosis of modified proteins (proteopathy) underlying social diseases (Report 1)StanishevskiyYa. M.StanishevskiyYa. M.

Department of Biomedical and Pharmaceutical Technology, associate professor

Department of Biomedical and Pharmaceutical Technology,  associate professor

stanyar@yandex.ruGritskovaI. A.GritskovaI. A.

Department of Chemical Technology of Macromolecular Compounds, professor

Department of Chemical Technology of Macromolecular Compounds, professor

noemail@neicon.ruKuzminaN. S.KuzminaN. S.

Laboratory of Immunological Diagnosis of Endocrine Diseases, head of the laboratory

Laboratory of Immunological Diagnosis of Endocrine Diseases, head of the laboratory

noemail@neicon.ruM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571РоссияM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Russian FederationMechnikov Research Institute of Vaccines and Sera, Moscow, 105064РоссияMechnikov Research Institute of Vaccines and Sera, Moscow, 105064Russian Federation201328062013838794Copyright © Stanishevskiy Y.M., Gritskova I.A., Kuzmina N.S., 20132013Stanishevskiy Y.M., Gritskova I.A., Kuzmina N.S.Stanishevskiy Y.M., Gritskova I.A., Kuzmina N.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/597

A new experimental technique for obtaining diagnostic test systems is introduced based on conjugates "polymer microsphere–bioligand" for rapid diagnosis of autoantibodies to the modified protein on example of thyroglobulin, which consists of preliminary blocking of aldehyde groups of polymer microspheres by glycine amine groups, and then of the covalent interaction between carboxyl group of glycine and the amino groups of thyroglobulin. This way of creating test systems has enhanced the sensitivity of the reaction of latex agglutination (RLA) by extending the boundaries of titer.

A new experimental technique for obtaining diagnostic test systems is introduced based on conjugates "polymer microsphere–bioligand" for rapid diagnosis of autoantibodies to the modified protein on example of thyroglobulin, which consists of preliminary blocking of aldehyde groups of polymer microspheres by glycine amine groups, and then of the covalent interaction between carboxyl group of glycine and the amino groups of thyroglobulin. This way of creating test systems has enhanced the sensitivity of the reaction of latex agglutination (RLA) by extending the boundaries of titer.

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The authors declare that there are no conflicts of interest present.