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8-Oxo-2’-deoxyguanosine – biomarker of the oxidative stress

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Abstract

Free radical mechanism of a cell damage is one of the universal non-specific pathogenic pathways in a cause of many diseases, including cancer, neurodegenerative diseases, atherosclerosis and aging. So in nuclear and mitochondrial DNA, guanine hydroxylation to 8-position gives 8­hydroxy­2'­deoxyguanosine (8­OH­dG) and 8­oxo­7,8­dihydro­2'­deoxyguanosine (8­oxo­dG). These substances are one of the predominant products of free radical­induced oxidative damages. They are usually been applied as biomarkers of oxidative stress and carcinogenesis. The direct oxidation of guanine or incorrect inclusion of 8-oxo-dGTP from the nucleotide pool by polymerases, lead to a lack of specificity of the base pairing in DNA, favoring mutagenesis. Firstly 8-oxo-dG has been described by H. Kasai and S. Nishimura in 1983. Since then, this damage has been widely measured in various tissues and body fluids as blood, urine, brain, liver, and others. Today 8-oxo-dG is already used not only as a marker of oxidative stress, but also as a tool for prognosis of diseases and results of applied therapy. Now many efforts are focused on developing the procedure of measurement of 8-oxo-dG content in tissues and body fluids. In this paper we also discuss the role of the 8-oxo-dG as a biomarker of oxidative stress and a predictor of diseases and results of the applied therapy.

About the Authors

T. S. Nevredimova
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


N. V. Marmiy
Московский государственный университет им. М.В. Ломоносова, Москва, 119991
Russian Federation


D. S. Esipov
Московский государственный университет им. М.В. Ломоносова, Москва, 119991
Russian Federation


O. V. Esipova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


V. I. Shvets
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


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For citation:


Nevredimova T.S., Marmiy N.V., Esipov D.S., Esipova O.V., Shvets V.I. 8-Oxo-2’-deoxyguanosine – biomarker of the oxidative stress. Fine Chemical Technologies. 2014;9(5):3-10. (In Russ.)

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