Development and scaling of a chromatographic purification technology for single-domain antibodies fused to the Fc fragment of human IgG1

D. S. Polyansky; V. V. Prokofiev; A. V. Samorukova; F. A. Shishkonakov; D. V. Vasiliev; E. I. Ryabova; A. A. Derkaev; A. I. Gosudarev; Yu. А. Efimova; D. V. Shcheblyakov; A. P. Karpov; I. B. Esmagambetov

Fine Chemical Technologies
Tonkie Khimicheskie Tekhnologii

ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)

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Polyansky D.S., Prokofiev V.V., Samorukova A.V., Shishkonakov F.A., Vasiliev D.V., Ryabova E.I., Derkaev A.A., Gosudarev A.I., Efimova Yu.А., Shcheblyakov D.V., Karpov A.P., Esmagambetov I.B. Development and scaling of a chromatographic purification technology for single-domain antibodies fused to the Fc fragment of human IgG1. Fine Chemical Technologies. 2026;21(2):188-211. https://doi.org/10.32362/2410-6593-2026-21-2-188-211. EDN: LAJHYG

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)

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About the Authors

D. S. Polyansky
MIREA – Russian Technological University
Russian Federation

Dmitry S. Polyansky, Assistant Professor, I.P. Alimarin Department of Analytical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

Scopus Author ID 57890564200

78, Vernadskogo pr., Moscow, 119454

V. V. Prokofiev
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Vladimir V. Prokofiev, Laboratory Research Assistant, Laboratory of Immunobiotechnology

Scopus Author ID 57300704700

18, Gamaleya ul., Moscow, 123098

A. V. Samorukova
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Alexandra V. Samorukova, Leading Technologist, Medgamal branch

18, Gamaleya ul., Moscow, 123098

F. A. Shishkonakov
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Fedor A. Shishkonakov, Technologist, Medgamal branch

18, Gamaleya ul., Moscow, 123098

D. V. Vasiliev
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Dmitry V. Vasiliev, Technologist, Medgamal branch

18, Gamaleya ul., Moscow, 123098

E. I. Ryabova
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Ekaterina I. Ryabova, Junior Researcher, Laboratory of Immunobiotechnology

Scopus Author ID 57301278100, ResearcherID AAE-7335-2022

18, Gamaleya ul., Moscow, 123098

A. A. Derkaev
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Artem A. Derkaev, Junior Researcher, Laboratory of Immunobiotechnology

Scopus Author ID 57381507000, ResearcherID AFU-7075-2022

18, Gamaleya ul., Moscow, 123098

A. I. Gosudarev
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Andrey I. Gosudarev, Head of the Department of Development and Scaling, Medgamal branch

18, Gamaleya ul., Moscow, 123098

Yu. А. Efimova
MIREA – Russian Technological University
Russian Federation

Yuliya A. Efimova, Cand. Sci. (Chem.), Assistant Professor, I.P. Alimarin Department of Analitical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

Scopus Author ID 25228417800

78, Vernadskogo pr., Moscow, 119454

D. V. Shcheblyakov
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Dmitry V. Shcheblyakov, Dr. Sci. (Biol.), Leading Researcher, Head of the Laboratory of Immunobiotechnology

Scopus Author ID 35073056900, ResearcherID E-5899-2014

18, Gamaleya ul., Moscow, 123098

A. P. Karpov
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Andrey P. Karpov, Cand. Sci. (Biol.), Chief Technologist, Medgamal branch

Scopus Author ID 22834845200

18, Gamaleya ul., Moscow, 123098

I. B. Esmagambetov
N.F. Gamaleya National Research Center for Epidemiology and Microbiology (The Gamaleya National Center), Ministry of Health of the Russian Federati
Russian Federation

Ilias B. Esmagambetov, Cand. Sci. (Biol.), Leading Researcher, Head of the Laboratory of Stromal Regulation of Immunity

Scopus Author ID 56120429700, ResearcherID E-3327-2014

18, Gamaleya ul., Moscow, 123098

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86, R-108, Vernadskogo pr., Moscow, 119571, Russian Federation
MIREA - Russian Technological University (RTU MIREA),
M.V. Lomonosov Institute of Fine Chemical Technologies

Managing Editor: Galina D. Seredina, Phone: +7 499 600-80-80 (#31288), E-mail: seredina@mirea.ru

Editor: Sofya M. Mazina, E-mail: mazina@mirea.ru

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