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Validation of a method for the quantitative determination of narcotic and psychotropic substances in urine by UHPLC–MS/MS

https://doi.org/10.32362/2410-6593-2022-17-3-253-267

Abstract

Objectives. To validate a new method for the quantitative determination of 31 potent and narcotic substances and their metabolites in urine that meets the requirements of ISO/IEC 17025 using a fast and highly sensitive method of chromato-mass spectrometry with a view to introducing such a method into the routine practice of the National Anti-Doping Laboratory of the Lomonosov Moscow State University (NADL MSU).

Methods. Urine samples soldered with standard solutions were analyzed using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS).

Results. Diagnostic precursor/ion-product pairs and collision energies were established to allow unambiguous identification of the analyzed substances. During sample preparation, hydrolysis conditions were optimized. Selectivity, linearity, limits of qualitative determination, limit of quantitative determination (established under the contract with the customer firm), matrix effect, and measurement uncertainty were defined. Systematized data grouped by classes of analytes are given in the final table.

Conclusions. The important advantages of the presented technique are the absence of complex and lengthy sample preparation, as well as the short time of the analysis method (about 10 min), which can significantly reduce duration along with labor and analysis costs. The addition of new analytes will ensure the versatility of the technique, as well as expanding its scope.

 

About the Authors

N. B. Savelieva
M.V. Lomonosov Moscow State University (NADL MSU)
Russian Federation

Nadezhda B. Savelieva, Chief Specialist of the Doping Control Department, National Anti-Doping Laboratory (Institute)

10-1, Elizavetinskii per., Moscow, 10500



G. V. Ishutenko
M.V. Lomonosov Moscow State University (NADL MSU)
Russian Federation

Grigory V. Ishutenko, Deputy Head of the Doping Control Department, National Anti-Doping Laboratory (Institute)

10-1, Elizavetinskii per., Moscow, 10500



A. V. Polosin
M.V. Lomonosov Moscow State University (NADL MSU)
Russian Federation

Andrey V. Polosin, Chief Specialist of the Doping Control Department, National Anti-Doping Laboratory (Institute)

10-1, Elizavetinskii per., Moscow, 10500



F. V. Radus
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

Fedor V. Radus, Assistant, I.P. Alimarin Department of Analitical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

86, Vernadskogo pr., Moscow, 119571



D. S. Polyansky
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

Dmitry S. Polyansky, Assistant, I.P. Alimarin Department of Analitical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

86, Vernadskogo pr., Moscow, 119571



S. A. Kurbatkin
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

Sergey A. Kurbatkin, Assistant, I.P. Alimarin Department of Analitical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

86, Vernadskogo pr., Moscow, 119571



Yu. A. Efimova
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

Yuliya A. Efimova, Cand. Sci. (Chem.), Assistant Professor, I.P. Alimarin Department of Analitical Chemistry, M.V. Lomonosov Institute of Fine Chemical Technologies

86, Vernadskogo pr., Moscow, 119571



P. V. Postnikov
M.V. Lomonosov Moscow State University (NADL MSU)
Russian Federation

Pavel V. Postnikov, Cand. Sci. (Chem.), Head of the Doping Control Department, National Anti-Doping Laboratory (Institute)

10-1, Elizavetinskii per., Moscow, 10500

RSCI SPIN-код 7251-9937



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Supplementary files

1. Linear calibration curve plot of hydromorphone
Subject
Type Исследовательские инструменты
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Indexing metadata ▾
  • Data on the validation of the method for the determination of narcotic, potent, and psychotropic substances in agreement with the ISO/IEC 17025-2019 requirements are presented.
  • For their reliable identification, diagnostic precursor/ion-product pairs and collision energies were established, hydrolysis conditions during sample preparation were optimized, selectivity, linearity, limits of qualitative and quantitative determinations, matrix effect, and measurement uncertainty were determined.
  • The important advantages of the presented technique are the absence of complex and lengthy sample preparation, as well as the short time of the analysis method, which can significantly reduce duration along with labor and analysis costs.

Review

For citations:


Savelieva N.B., Ishutenko G.V., Polosin A.V., Radus F.V., Polyansky D.S., Kurbatkin S.A., Efimova Yu.A., Postnikov P.V. Validation of a method for the quantitative determination of narcotic and psychotropic substances in urine by UHPLC–MS/MS. Fine Chemical Technologies. 2022;17(3):253-267. https://doi.org/10.32362/2410-6593-2022-17-3-253-267

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)