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Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications

https://doi.org/10.32362/2410-6593-2021-16-5-414-425

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Abstract

Objectives. This paper presents data on the development and study of the structural properties of iron-doped crystalline silicon (nc-Si/SiOx/Fe) nanoparticles obtained using the plasma-chemical method for application in magnetic resonance imaging diagnostics and treatment of oncological diseases. This work aimed to use a variety of analytical methods to study the structural properties of nc-Si/SiOx/Fe and their colloidal stabilization with citrate anions for in vivo applications.
Methods. Silicon nanoparticles obtained via the plasma-chemical synthesis method were characterized by laser spark emission spectroscopy, atomic emission spectroscopy, Fouriertransform infrared spectroscopy, and X-ray photoelectron spectroscopy. The hydrodynamic diameter of the nanoparticles was estimated using dynamic light scattering. The toxicity of the nanoparticles was investigated using a colorimetric MTT test for the cell metabolic activity. Elemental iron with different Fe/Si atomic ratios was added to the feedstock during loading.
Results. The particles were shown to have a large silicon core covered by a relatively thin layer of intermediate oxides (interface) and an amorphous oxide shell, which is silicon oxide with different oxidation states SiOx (0 ≤ x ≤ 2). The samples had an iron content of 0.8–1.8 at %. Colloidal solutions of the nanoparticles stabilized by citrate anions were obtained and characterized. According to the analysis of the cytotoxicity of the modified nanosilicon particles using monoclonal K562 human erythroleukemia cells, no toxicity was found for cells in culture at particle concentrations of up to 5 µg/mL.
Conclusions. Since the obtained modified particles are nontoxic, they can be used in in vivo theranostic applications.

About the Authors

K. I. Rozhkov
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

 Postgraduate Student, I.P. Alimarin Department of Analytical Chemistry

86, Vernadskogo pr., Moscow, 119571, Russia



E. Y. Yagudaeva
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation

 Cand. Sci. (Chem.), Senior Researcher

Miklukho-Maklaya ul., 16/10, Moscow, 117997, Russia



S. V. Sizova
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation

 Cand. Sci. (Chem.), Researcher

Miklukho-Maklaya ul., 16/10, Moscow, 117997, Russia



M. A. Lazov
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

 Assistant, I.P. Alimarin Department of Analytical Chemistry

86, Vernadskogo pr., Moscow 119571, Russia



E. V. Smirnova
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation

 Cand. Sci. (Biol.), Researcher

Miklukho-Maklaya ul., 16/10, Moscow, 117997, Russia



V. P. Zubov
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies); Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation

 Dr. Sci. (Chem.), Professor, S.S. Medvedev Department of Chemistry and Technology of HighMolecular Compounds, Principal Researcher

86, Vernadskogo pr., Moscow, 119571, Russia

Miklukho-Maklaya ul., 16/10, Moscow, 117997, Russia



A. A. Ischenko
MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation

 Dr. Sci. (Chem.), Professor, I.P. Alimarin Department of Analytical Chemistry

86, Vernadskogo pr., Moscow, 119571, Russia



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Supplementary files

1. Fourier-transform infrared spectrum of the studied nc-Si/SiOx/Fe sample (0.3 mass % in КBr)
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2. This is to certify that the paper titled Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications commissioned to us by Kirill I. Rozhkov, Elena Y. Yagudaeva, Svetlana V. Sizova, Michael A. Lazov, Evgeniya V. Smirnova, Vitaliy P. Zubov and Anatoliy A. Ischenko has been edited for English language and spelling by Enago, an editing brand of Crimson Interactive Inc.
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  • A technique for introducing iron into the shell of silicon nanoparticles obtained via the plasma-chemical method was developed.
  • The possibility of obtaining the stable aqueous solutions of silicon nanoparticles by modifying their surfaces with citrate anions was shown.
  • Analysis of the cytotoxicity of nanoparticles modified by citrate anions using monoclonal cells of human erythroleukemia K562 showed no toxicity for cells in culture at a particle concentration of up to 5 μg/mL.
  • The obtained citrate anions modified iron-doped nc-Si particles can be recommended for the bioimaging, for example, in MRI diagnostics.

For citation:


Rozhkov K.I., Yagudaeva E.Y., Sizova S.V., Lazov M.A., Smirnova E.V., Zubov V.P., Ischenko A.A. Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications. Fine Chemical Technologies. 2021;16(5):414-425. https://doi.org/10.32362/2410-6593-2021-16-5-414-425

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)