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New approaches for the standardization of the Monarda fistulosa herb

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Objectives. The Monarda fistulosa herb of the Lamiaceae family is particularly interesting among essential oils from medicinal plants that have a wide spectrum of pharmacological activities. However, information regarding some of its flavonoids, which are found in the essential oil, is controversial. Inaccuracies in identification of the chemical composition of the herb have led to several different standardization approaches, which are cumbersome. To establish a uniform classification, here, we present confirmation for new approaches for the standardization of the Monarda fistulosa herb.

Methods. Silica gel column chromatography was used to extract the flavonoids. Identification was based on ultraviolet spectroscopy, nuclear magnetic resonance spectroscopy, mass spectrometry, and acidic hydrolysis. The quality of the proposed quantitation methodology for total flavonoids was assessed by differential spectrophotometry at 394 nm, in isorhoifolin equivalent.

Results. We have verified new approaches for the standardization of the Monarda fistulosa herb. The approaches can determine the authenticity of the herb by detecting monoterpene phenols and flavonoids that have diagnostic value. We also developed a technique for quantitation of the total flavonoids.

Conclusions. We investigated the possibility of establishing the authenticity of the Monarda fistulosa herb based on the diagnostically significant flavonoids, isorhoifolin and linarin.

About the Authors

V. A. Kurkin
Samara State Medical University
Russian Federation

Vladimir A. Kurkin, Dr. of Sci. (Pharmaceuticals), Professor

89, Chapaevskaya ul., Samara, 443099

A. S. Tsibina
Samara State Medical University
Russian Federation

Anastasiya S. Tsibina, Postgraduate Student

89, Chapaevskaya ul., Samara, 443099


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Supplementary files

1. Chromatogram of the water-alcohol extract from Monarda fistulosa in the chloroform–ethanol–water (26 : 16 : 3) system. Detection in visible light after treatment with the alkaline solution of diazobenzenesulfonic acid. Designations: 1 – extract from Monarda fistulosa; 2 – isorhoifolin; 3 – linarin; 4 – rutin; 5 – thymol.
Type Исследовательские инструменты
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2. This is to certify that the paper titled New approaches for the standardization of the Monarda fistulosa herb commissioned to us by Vladimir A. Kurkin, Anastasiya S. Tsibina has been edited for English language and spelling by Enago, an editing brand of Crimson Interactive Inc.
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  • From the herb Monarda fistulosa L., for the first time, we isolated the flavonoids isorhoifolin (7-O-rutinoside of apigenin) and linarin (7-O-rutinoside of acacetin), which were identified using of 1H-NMR-, 13C-NMR-, UV-spectroscopy, mass spectrometry, and the results of chemical transformations.
  • The expediency of the determination of the authenticity of the Monarda fistulosa herbs was achieved by detecting the diagnostically significant flavonoids, isorhoifolin, linarin, and monoterpene phenols of carvacrol and thymol.
  • The spectral characteristics of the UV spectrum of the water–alcohol extraction from the herb Monarda fistulosa were due by the flavones isorhoifolin and linarin.
  • A method of quantitative determination of the total flavonoids in the Monarda fistulosa herb by differential spectrophotometry at a wavelength of 394 nm in terms of isorhoifolin was developed.

For citation:

Kurkin V.A., Tsibina A.S. New approaches for the standardization of the Monarda fistulosa herb. Fine Chemical Technologies. 2020;15(4):30-38.

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