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Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.

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Abstract

Recombinant chimeric constructions RTA-DHFR and RTВ-DHFR consisting of ricin Aand B-subunits genes (RTA and RTB) and dihydrofolate reductase (DHFR) gene were cloned and expressed in Escheriсhia coli. Antigenic properties of RTA and RTB retained in chimeric proteins RTA-DHFR and RTВ-DHFR.

About the Authors

M. А. Gracheva
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation


Z. М. Galushkina
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation


N. N. Poletaeva
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation


L. V. Verhovskaya
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation


V. G. Lunin
ГУ НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation


V. I. Shvets
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


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For citation:


Gracheva M.А., Galushkina Z.М., Poletaeva N.N., Verhovskaya L.V., Lunin V.G., Shvets V.I. Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.. Fine Chemical Technologies. 2007;2(6):40-47. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)