Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.
Abstract
About the Authors
M. А. GrachevaRussian Federation
Z. М. Galushkina
Russian Federation
N. N. Poletaeva
Russian Federation
L. V. Verhovskaya
Russian Federation
V. G. Lunin
Russian Federation
V. I. Shvets
Russian Federation
References
1. Антонов, Н. С. Химическое оружие на рубеже двух столетий / Н. С. Антонов. – М. : Прогресс, 1994. – C. 108–111.
2. Doan, L. G. Ricin: mechanism of toxity, clinical manifestations, and vaccine development / L. G. Doan // J. Toxicol. Clin. Toxicol. – 2004. - Vol. 42, № 2. – P. 201–208.
3. Bigalke, H. Medical aspects of toxin weapon / H. Bigalke, A. Rummel // Toxicology. – 2005. – Vol. 214. – P. 210–220.
4. Olsnes, S. Ricin / S. Olsnes, J. V. Kozlov // Toxicon. – 2001. – Vol. 39, № 11. – P. 1723–1728.
5. Козлов, Ю. В. Рибосом-инактивирующие лектины растений / Ю. В. Козлов, О. Ю. Сударкина, А. Г. Курманова // Мол. Биол. – 2006. – Т. 40, № 4. – С. 711–723.
6. Ricin: current understanding and prospects for an antiricin vaccine / C. J Marsden [et al.] // Expert Rev. Vaccines. – 2005. – Vol. 4, № 2. – P. 229–237.
7. Improved formulation of a recombinant ricin A-chain vaccine increases its stability and effective antigenicity / J. H. Carra [et al.] // Vaccine. – 2007. – Vol. 25, № 21. – P. 4149–4158.
8. Mutational analysis of the galactose binding ability of recombinant ricin В chain / R. Wales [et al.] // J. Biol.Chem. – 1991. – Vol. 266, № 29. – P. 19172–19179.
9. The effects of N-glycosylation on the lectin activity of recombinant ricin B chain / P. T. Richardson [et al.] // Carbohydr. Res. – 1991. – Vol. 213. – P. 19–25.
10. Terpe, K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems / K. Terpe // Appl. Microbiol. Biotechnol. – 2003. – Vol. 60, № 5. – P. 523–533.
11. Henco, K. The QIAexpressionist / K. Henco // DIAGEN GmbH, QIAGEN Inc. – 1991. – P. 1–55.
12. Дорохов, Д.Б. Быстрая и экономичная технология RAPD анализа растительных геномов / Д. Б. Дорохов, Э. Клоке // Молекулярная генетика. – 1997. – Т. 33, № 4. – С. 443–450.
13. Маниатис, Т. Методы генетической инженерии. Молекулярное клонирование / Т. Маниатис, Э. Фрич, Д. Сэмбрук. – М. : Мир, 1984.
14. Sanger, F. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase / F. Sanger, A.R. Coulson // J. Mol. Biol. – 1975. – Vol. 94, № 3. – P. 441–446.
15. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / U.K. Laemmli // Nature. – 1970. – Vol. 227, № 5259 – P. 680–682.
16. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / M. M. Bradford // Anal. Biochem. – 1976. – Vol. 72. – P. 248–254.
Review
For citations:
Gracheva M.А., Galushkina Z.М., Poletaeva N.N., Verhovskaya L.V., Lunin V.G., Shvets V.I. Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli. Fine Chemical Technologies. 2007;2(6):40-47. (In Russ.)