Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.
Abstract
Recombinant chimeric constructions RTA-DHFR and RTВ-DHFR consisting of ricin Aand B-subunits genes (RTA and RTB) and dihydrofolate reductase (DHFR) gene were cloned and expressed in Escheriсhia coli. Antigenic properties of RTA and RTB retained in chimeric proteins RTA-DHFR and RTВ-DHFR.
About the Authors
M. А. Gracheva
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation
Russian Federation
Z. М. Galushkina
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation
Russian Federation
N. N. Poletaeva
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation
Russian Federation
L. V. Verhovskaya
У НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation
Russian Federation
V. G. Lunin
ГУ НИИ Эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН
Russian Federation
Russian Federation
V. I. Shvets
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Russian Federation
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Review
For citations:
Gracheva M.А., Galushkina Z.М., Poletaeva N.N., Verhovskaya L.V., Lunin V.G., Shvets V.I. Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli. Fine Chemical Technologies. 2007;2(6):40-47. (In Russ.)
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