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Test system creation for analysis of template switching effect during a reverse transcription in vitro

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Abstract

We investigated an in vitro system for the analysis of template switching effect of RNA-dependent DNA-polymerase during reverse transcription reaction. An RT-PCR-based approach was realized. An RNA sample was used as a template. The RNA contains a region with a stable secondary structure flanked by two homologous sequences. The described system can find use for testing overall performance of new and existing forms of the reverse transcriptase.

About the Authors

A. Y. Komkov
Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН
Russian Federation


Y. B. Lebedev
Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН
Russian Federation


V. I. Shvets
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


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For citation:


Komkov A.Y., Lebedev Y.B., Shvets V.I. Test system creation for analysis of template switching effect during a reverse transcription in vitro. Fine Chemical Technologies. 2011;6(6):77-83. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)