.Study of adsorption of factor vVIIIVFW complex on ion-exchange supports.
Abstract
We investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best results. The adsorption was performed by a batch-method when mixing at the room t0 and a residual FVIII and vWF-content was measured in the supernatant. The maximum binding capacity was estimated 188 IU FVIII /g and 2309 IU vWF /g for Fractogel EMD TMAE (M) and 134 IU FVIII /g and 1935 IU vWF /g for Fractogel EMD TMAE Hicap (M). The results obtained may be used for development of the method of purifying a FVIII/vWF complex using ion-exchange chromatography.
About the Authors
M. A. Azighirova
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
T. L. Dereza
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
O. G. Kuturova
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
T. A. Zipileva
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
References
1. A novel mechanism of factor VIII protection by von Willebrand factor from activated protein C-catalyzed inactivation/ K. Nogami [et al.] // Blood. - 2002. - Vol. 99, № 11.- P. 3993-3998.
2. Factor VIII inhibitors: role of von Willebrand factor on the uptake of factor VIII by dendritic cells / S.V Kaveri [et al.] // Haemophilia. - 2007. - Vol. 13. - P. 61-64.
3. Goudemand, J. Inhibitor development in haemophilia A: the role of von Willebrand factor/factor VIII concentrates / J. Goudemand // Haemophilia. - 2007. - Vol. 13. - P. 47-51.
4. Gringeri, A. vWF/FVII concentrates in high-risk immunotolerance: the RESIST study / A. Gringeri // Haemophilia. - 2007. - Vol. 13. - P. 73-77.
5. Fibronectin in frozen products of human plasma / S. Mitrovic [et al]. //Abstr. of VI Regional European congress of the ISBT. - 1999. - P. 67.
6. Pat. 6,541,518 US, МПК А 61 К 031/19. Enhanced production of safe plasma preparations / E.Shanbrom. - № 778681 ; заявлено 02.07.01 ; опубл. 01.04.03.
7. Ажигирова, М. А. Влияние условий получения криопреципитата на его состав / М. А. Ажигирова, Т. Л. Дереза, О. Г. Кутюрова, Т. А. Ципилева // Гематол. и трансфузиол. - 2008. - Т. 53, № 1. - С. 12-14.
8. Janson, J. C. Protein purification / J. C Janson, L.Ryden. - N.Y. : Wiley-Liss, 1998. - 166 p.
9. Бутинас, С. Свертывание крови / С. Бутинас, К.Г. Манн // Биохимия. - 2002. - Т. 67, № 1. - С. 5-15.
10. Furlan, M. Von Willebrand factor: molecular size and functional activity / M. Furlan // Ann. Hematol. - 1996. - Vol. 72. - P. 341-348.
11. Progress in large-scale purification of factor VIII/von Willebrand factor concentrates using ion-exchange chromatography/ F. Mori, I. Nardini, P. Rossi, C. Farina // Vox Sang. - 2008. - Vol. 95. - P. 298-307.
12. European Pharmacopeia, 4th ed. - 2002. - P. 166-168.
13. Sarji, K. E. Nature of von Willebrand factor: a new assay and a specific inhibitor / K. E. Sarji // Proc. Natl. Acad. Sci. USA. - 1974. - Vol. 71. - P. 2937-2941.
14. Государственная фармакопея РФ / изд. XII, ч. 1. - М.: Научный центр экспертизы средств медицинского применения, 2008. - С. 109-110.
15. Банержи, А. Медицинская статистика понятным языком: вводный курс / Пер. с англ. под ред. В. П. Леонова. - М. : Практическая медицина, 2007. - 287с.
Review
For citations:
Azighirova M.A., Dereza T.L., Kuturova O.G., Zipileva T.A. .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. Fine Chemical Technologies. 2009;4(5):20-24. (In Russ.)
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