.Study of adsorption of factor vVIIIVFW complex on ion-exchange supports.
Abstract
We investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best results. The adsorption was performed by a batch-method when mixing at the room t0 and a residual FVIII and vWF-content was measured in the supernatant. The maximum binding capacity was estimated 188 IU FVIII /g and 2309 IU vWF /g for Fractogel EMD TMAE (M) and 134 IU FVIII /g and 1935 IU vWF /g for Fractogel EMD TMAE Hicap (M). The results obtained may be used for development of the method of purifying a FVIII/vWF complex using ion-exchange chromatography.
About the Authors
M. A. Azighirova
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
T. L. Dereza
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
O. G. Kuturova
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
T. A. Zipileva
Учреждение Российской академии медицинских наук
Russian Federation
Russian Federation
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Review
For citations:
Azighirova M.A., Dereza T.L., Kuturova O.G., Zipileva T.A. .Study of adsorption of factor vVIIIVFW complex on ion-exchange supports. Fine Chemical Technologies. 2009;4(5):20-24. (In Russ.)
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