Preview

Fine Chemical Technologies

Advanced search

Liposomes containing dexamethasone: preparation, characteristics and application in ophthalmology

Full Text:

Abstract

Glucocorticoid dexamethasone (DM) liposomes of various lipid compositions were obtained. DM was used in insoluble form and in water-soluble form as disodium salt of dexamethasone phosphate (DMP). Correspondence between the drug inclusion percent and the structure of liposomes, and its solubility in water was studied. Lipids were shown to allow DM solubilization at concentration exceeding its solubility in water 9.3 times. Using repeated gel-chromatography, release of medicinal substances from liposomes was determined for all the samples of liposomes containing both DМP and DM. It was thus shown that up to 87% of the included substance remained in the liposomes. Biocompatibility of «empty» liposomes and liposomes containing a glucocorticoid with rabbit eyeball tissues was studied after their endovitreal incorporation. Dipalmitoylphosphatidylcholine liposomes were demonstrated not to cause inflammatory reaction after introduction in this way. Distribution of the liposomes in tissues of the rabbit eye posterior chamber was determined. It was shown that the liposomes after endovitreal introduction were distributed in various layers of retina.

About the Authors

N. L. Leparskаya
Московский научно-исследовательский институт глазных болезней им. Гельмгольца Росмедтехнологий
Russian Federation


G. M. Sоrokoumоva
M.V. Lomonosov Moscow University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


Yu. V. Sycheva
M.V. Lomonosov Moscow University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


I. P. Horoshilovа-Maslova
Московский научно-исследовательский институт глазных болезней им. Гельмгольца Росмедтехнологий
Russian Federation


A. P. Kaplun
M.V. Lomonosov Moscow University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


I. I. Kereev
Научно-исследовательский институт физико-химической биологии им. А.Н. Белозерского МГУ им. М.В. Ломоносова
Russian Federation


R. A. Gundorova
МИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86
Russian Federation


V. V. Neroev

Russian Federation


V. I. Shvets
M.V. Lomonosov Moscow University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571


References

1. Maurice D.M., Mishima S. Ocular pharmacokinetics // In: Handbook of Experimental Pharmacology / Ed. M.L. Sears. - Berlin-Heidelberg: Springer Verlag, 1984. V. 69. P. 16-119.

2. Meisner D., Mezei M. Liposome ocular delivery systems // Adv. Drug Deliv. Rev. 1995. V. 16. P. 75-93.

3. Швец В.И., Каплун А.П., Краснопольский Ю.М., Степанов А.Е., Чехонин В.П. От липосом семидесятых к нанобиотехнологии XXI века // Рос. нанотехнологии. 2008. Т. 3. Вып. 11-12. С. 52-66.

4. Майчук Ю.Ф. Иммунокорригирующая терапия при воспалительных заболеваниях глаз // Офтальмолог. 2008. № 1 (июль-август). С. 20.

5. Christie J.G., Kompella U.B. Ophtalmic light sensitive nanocarrier systems // Drug Discov. Today. 2008. V. 13. P. 124-134.

6. McGhee C.N.J., Dean S., Danesh-Meyer H. Locally administered ocular corticosteroids // Drug Safety. 2002. V. 25. № 1. P. 33-55.

7. Справочник ВИДАЛЬ. - М.: АстраФармСервис, 1999. 1520 с.

8. Кленин В.И., Щеголев С.Ю., Лаврушин В.И. Характеристические функции светорассеяния дисперсных систем. - Саратов: Изд-во Саратовского университета, 1977. 176 c.

9. Ghee C.N., Watson D.G., Midgley J.M. Penetration of synthetic corticosteroids into human aqueous humor // Eye. 1990. V. 4. № 3. P. 526-530.


For citation:


Leparskаya N.L., Sоrokoumоva G.M., Sycheva Yu.V., Horoshilovа-Maslova I.P., Kaplun A.P., Kereev I.I., Gundorova R.A., Neroev V.V., Shvets V.I. Liposomes containing dexamethasone: preparation, characteristics and application in ophthalmology. Fine Chemical Technologies. 2011;6(2):37-42. (In Russ.)

Views: 98


ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)