Inhibitory action of exogenous cardiolipin on the bacterial type i and ii dna topoisomerases in vitro
Abstract
The influence of empty liposomes composed of cardiolipin (lipid which is a component of the bacterial cytoplasmic membrane and eukaryotic internal mitochondrial membrane) on Escherichia coli topoisomerase I and DNA gyrase (topoisomerase II) in vitro was investigated. Cardiolipin was found to inhibit the reaction of DNA relaxation catalyzed by topoisomerase I and both reactions catalyzed by DNA gyrase, namely DNA relaxation (in the absence of ATP) and DNA supercoiling (in the presence of ATP). On the basis of the results obtained along with the bactericidal action of cardiolipin on Mycobacterium tuberculosis and Escherichia coli earlier found by us we suppose that topoisomerases could be the targets of cardiolipin.
Keywords
cardiolipin,
inhibitory action,
bacterial enzymes,
topoisomerase I,
DNA gyrase,
relaxed DNA,
supercoiled DNA
579.842.11
About the Authors
Yu. L. Mikulovich
МИТХТ им. М.В. Ломоносова
Russian Federation
Russian Federation
G. М. Sorokoumova
МИТХТ им. М.В. Ломоносова
Russian Federation
Russian Federation
А. А. Selishcheva
МИТХТ им. М.В. Ломоносова
Russian Federation
Russian Federation
V. I. Shvets
МИТХТ им. М.В. Ломоносова
Russian Federation
Russian Federation
References
1. Drlica K., Zhao X. DNA gyrase, topoisomerase IV, and the 4-quinolones // Microbiol. Mol. Biol. Rev. 1997. V. 61. P. 377-392.
2. Bailly C. Topoisomerase I poisons and suppressors as anticancer drugs // Cur. Med. Chem. 2000. V. 7. P. 39-58.
3. Андреевская С.Н., Смирнова Т.Г., Жогина Ю.А. Смирнова Д.И, Микулович Ю.Л., Сорокоумова Г.М., Черноусова Л.Н., Селищева А.А., Швец В.И. Влияние экзогенного кардиолипина на рост и жизнеспособность Mycobacterium tuberculosis H37Rv in vitro // Доклады академии наук. 2010. Т. 434. № 5. С. 705-708.
4. Смирнова Т.Г., Микулович Ю.Л., Андреевская С.Н., Сорокоумова Г.М., Черноусова Л.Н., Селищева А.А., Швец В.И. Лизопроизводные кардиолипина подавляют жизнеспособность чувствительного и резистентного штаммов Mycobacterium tuberculosis // Биофарм. журн. 2011. Т. 3. № 2. С. 19-27.
5. Lewis R.N.A.H., McElhaney R.N. The physicochemical properties of cardiolipin bilayers and cardiolipin-containing lipid membranes // Biochim. Biophys. Acta. 2009. v. 1788. p. 2069-2079.
6. Wickner W., Kornberg A. A holoenzyme form of deoxyribonucleic acid polymerase III // J. Biol. Chem. 1974. V. 249. P. 6244-6249.
7. Arias-Cartin R., Grimaldi S., Pommier J., Lanciano P., Schaefer C., Arnoux P., Giordano G., Guigliarelli B., Magalon A. Cardiolipin-based respiratory complex activation in bacteria // Proc. Natl. Acad. Sci. USA. 2011. V. 108. P. 7781-7786.
8. Gold V.A.M., Robson A., Bao H., Romantsova Tatyana, Duong F., Collinson I. The action of cardiolipin on the bacterial translocon // Proc. Natl. Acad. Sci. USA. 2010. V. 107. P. 10044-10049.
9. Charalambous K., Miller D., Curnow P., Booth P.J. Lipid bilayer composition influences small multidrug transporters // BMC Biochem. 2008. V. 9. P. 31-43.
10. Mileykovskaya E., Dowhan W. Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange // J. Bacteriol. 2000. V. 182. P. 1172-1175.
11. Sekimizu K. Interactions between DNA replication-related proteins and phospholipid vesicles in vitro // J. Chem. & Phys. Lipids. 1994. V. 73. P. 223-230.
12. Mileykovskaya E., Fishov I., Fu X., Corbin B.D., Margolin W., Dowhan W. Effects of phospholipid composition on MinD-membrane interactions in vitro and in vivo // J. Biol. Chem. 2003. V. 278. P. 22193-22198.
13. Mizushima T., Natori S., Sekimizu K. Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids // Biochem. J. 1992. V. 285. P. 503-506.
14. Бугреев Д.В., Невинский Г.А. Структура и механизм действия ДНК-топоизомераз IА-типа // Успехи биол. химии. 2009. Т. 49. С.129-158.
15. Nagaraja V. Regulation of DNA topology in mycobacteria // Cur. Sci. 2004. V. 86. № 1. Р. 135-140.
16. Tamura H., Ikegami Y., Ono K., Sekimizu K., Andoh T. Acidic phospholipids directly inhibit DNA binding of mammalian DNA topoisomerase I // FEBS. 1990. V. 261. № 1. P. 151-154.
17. Tabary X., Moreau N., Dureuil C., Le Goffic F. Effect of DNA gyrase inhibitors pefloxacin, five other quinolones, novobiocin, and clorobiocin on Escherichia coli topoisomerase I // Antimicrob. Agents Chemother. 1987. V. 31. P. 1925-1928.
18. Сорокоумова Г.М., Андреевская С.М., Смирнова Т.Г., Петрова Е.Е., Жогина Ю.А., Калашникова Т.Ю., Черноусова Л.Н., Селищева А.А., Швец В.И. Влияние липосом различного липидного состава на рост Mycobacterium tuberculosis H37Rv in vitro // Бюлл. эксп. биол. и мед. 2009. Т. 148. № 11. С. 550-553.
19. Сорокоумова Г.М., Селищева А.А., Каплун А.П. Фосфолипиды. Методы их выделения, обнаружения и изучения физико-химических свойств липидных дисперсий в воде: учебн. пособие по биоорган. химии. - М.: ИПЦ МИТХТ, 2000. 68 с. 20. QIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga Kits for purification of ultrapure, transfection grade plasmid DNA. - QIAGEN® Plasmid Purification Handbook. 2005. 52 р.
Review
For citations:
Mikulovich Yu.L., Sorokoumova G.М., Selishcheva А.А., Shvets V.I. Inhibitory action of exogenous cardiolipin on the bacterial type i and ii dna topoisomerases in vitro. Fine Chemical Technologies. 2012;7(4):58-62. (In Russ.)
Views: 179
Email this article 