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Preparation of spherical microparticles from biodegradable natural and synthetic polymers for their application in regenerative medicine

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Abstract

We have studied methods for preparing biodegradable microparticles for regenerative medicine based on chitosan, polylactic acid, collagen and gelatin. The main methods of forming particles become emulsification, coacervation and gelation. These methods can achieve the necessary parameters of the product such as size, porosity and shape by varying process parameters such as temperature, stirring speed and the concentration of solutions. For drying the microparticles vacuum freeze drying was used, which allows to maintain the spherical shape of the particles after drying. The resulting microparticles were examined and photographed using a scanning electron microscope. To determine the cytotoxicity of the microparticles their cell growth and attachment were studied in vitro.

About the Authors

M. G. Gordienko
Mendeleev University of Chemical Technology of Russia, Moscow, 125480
Russian Federation


T. N. Somov
Mendeleev University of Chemical Technology of Russia, Moscow, 125480
Russian Federation


Y. S. Yusupova
Mendeleev University of Chemical Technology of Russia, Moscow, 125480
Russian Federation


N. I. Chupikova
LLC «Cellular Systems», Moscow, 125212
Russian Federation


N. V. Menshutina
Mendeleev University of Chemical Technology of Russia, Moscow, 125480
Russian Federation


References

1. Prafulla C., Yoo J.J., Lee S.J. Biomaterials in Regenerative Medicine: Challenges in Technology Transfer from Science to Process Development / In: Translational Regenerative Medicine. 2015. P. 151-157.

2. Chau D.Y.S., Agashi K., Shakesheff K.M. // Materials Sci. & Technol. 2008. V. 24. P. 1031-1044.

3. Whittlesey K.J., Shea L.D. // Exp. Neurology. 2004. V. 190. Р. 1-16.

4. Watts P.J., Davies M.C., Melia C.D. // Crit. Rev. in Therapeutic Drug Carrier Systems. 1990. V. 7. Р. 235-259.

5. Ogawa Y., Yamamoto M., Takada S., Okada H., Shimamoto T. // Chem. Pharm. Bull. 1988. V. 36. P. 1502-1507.

6. Hou Q., De Bank P.A., Shakesheff K.M. // J. Mater. Chem. 2004. V. 14. P. 1915-1923.

7. Masters K. Spray Drying Handbook: 4th ed. Bath: The Pitman Press Pitman, 1985. 696 p.

8. Wagenaar B.W., Müller B.W. // Biomaterials. 1994. V. 15. P. 49-54.

9. Berkland C., Kim K., Pack D.W. // J. Contr. Release. 2001. V. 73. P. 59-74.

10. Jain R.A. // Biomaterials. 2000. V. 21. P. 2475-2490.

11. Jalil R., Nixon J.R. // J. Microencapsulation.1990. V. 7. Р. 297-325.

12. Vasir J.K., Tambwekar K., Garg S. // Int. J. Pharm. 2003. V. 255. P. 13-32.

13. Wu T.J., Huang H.H., Hsu Y.M., Lyu S.R., Wang Y.J. // Biotechnol. Bioeng. 2007. V. 98. P. 578-585.

14. Zimmermann H., Shirley S. G., Zimmermann U. // Curr. Diabetes Rep. 2007. V. 7. P. 314-320.

15. Reis P.C., Neufeld R.J., Vilela S., Ribeiro A.J., Veiga F. // J. Microencapsulation. 2006. V. 23. P. 245-257.

16. Haug I.J., Draget K.I. Gelatin / In: Handbook of Food Proteins. 2011. P. 92-115.

17. Стариков Д.Г., Сомов Т.Н. // Успехи в химии и хим. технологии. 2013. Т. XXVII. № 1. C. 74-79.


For citation:


Gordienko M.G., Somov T.N., Yusupova Yu.S., Chupikova N.I., Menshutina N.V. Preparation of spherical microparticles from biodegradable natural and synthetic polymers for their application in regenerative medicine. Fine Chemical Technologies. 2015;10(5):66-76. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)