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Effect of melatonin on formation of 8-oxo-2’-deoxyguanosine under fenton reaction conditions

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Abstract

In this paper we studied the effect of melatonin at three concentrations on the formation of 8-oxo-2'-dioxyguanosine (8-oxo-dG) under the conditions of the Fenton reaction. Reproducible kinetic curve of 8-oxo-dG accumulation in this reaction was obtained by the method of reversedphase high performance liquid chromatography with amperometric detection. When adding melatonin in all three concentrations studied, there was a noticeable change in the shape of the kinetic curve with a significant decrease in the amount of detected 8-oxo-dG in most of its control points. This suggests the melatonin inhibition of 8-oxo-dG formation in the reaction and confirms the hypothesis of a direct, non-hormonal, antioxidant effect of melatonin. However, the dependence of the inhibitory effect of melatonin on its concentration is not a straight line. The higher of the studied concentrations of melatonin (60 mg/ml) gave the least pronounced effect.

About the Authors

N. V. Marmiy
M.V. Lomonosov Moscow State University, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Biology faculty


T. S. Nevredimova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


D. S. Esipov
M.V. Lomonosov Moscow State University, 86, Vernadskogo pr., Moscow 119571
Russian Federation
Biology faculty


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For citation:


Marmiy N.V., Nevredimova T.S., Esipov D.S. Effect of melatonin on formation of 8-oxo-2’-deoxyguanosine under fenton reaction conditions. Fine Chemical Technologies. 2015;10(1):50-55. (In Russ.)

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