Preview

Fine Chemical Technologies

Advanced search

Study of polysialated recombinant human insulin

Full Text:

Abstract

The method of structure determination of the recombinant glycosylated peptides and proteins has been elaborated by the example of the gene-spliced human insulin.

Keywords


543.544.5.068.7, 543.645.6

About the Authors

M. N. Korchagnikova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


I. V. Nazimov
Институт биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН
Russian Federation


Yu. M. Glubokov
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


V. V. Bezuglov
Институт биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН
Russian Federation


References

1. Byrne, B. Sialic acids: carbohydrate moieties that influence the biological and physical properties of biopharmaceutical proteins and living cells / B. Byrne, G. G. Donohoe, R. O'Kennedy // Drug Discovery Today. - 2007. - Vol. 12. - P. 319-326.

2. Polysialic acids: potential in drug delivery / G. Gregoriadis [et al.] // FEBS Lett. - 1993. - Vol. 315. - P. 271-276.

3. Valuev, L. New possibilities for biospecific chromatography / L. Valuev, N. A. Platé // Appl. Biochem. and Microbiol. - 2003. - Vol. 39, № 4. - P. 22-42.

4. Аналитическая биотехнология рекомбинантных пептидов и белков. Анализ чистот, состава и структуры инсулинов человека, свиньи и крупного рогатого скота / Н. В. Сергеев, И. В. Назимов, В. Г. Гавриков, А. И. Мирошников // Биоорган. химия. - 2000. - Т. 26, № 1. - С. 25-30.

5. Grau, U. Fingerprint analysis of insulin and proinsulins / U. Grau // Diabetus. - 1985. - Vol. 33. - P. 1174-1180.

6. Дарбре, А. Практическая химия белка / А. Дарбре. - М. : Мир, 1989. - 623 с.


For citation:


Korchagnikova M.N., Nazimov I.V., Glubokov Yu.M., Bezuglov V.V. Study of polysialated recombinant human insulin. Fine Chemical Technologies. 2009;4(2):77-79. (In Russ.)

Views: 48


ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)