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Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III

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Abstract

This paper reports the chromatographic properties of affinity resins in respect of protein antithrombin III. The capacity of resin Toyopearl AF-Heparin was found to be higher than that of resin of Heparin-Sepharose FF. Sorption was carried out by the batch method at room temperature and constant stirring. The specific activity of antithrombin III was estimated by the amidolytic method with the use of a chromogenic substrate. The obtained results can be used for the development of column chromatographic method of isolation of antithrombin III from human plasma.

About the Authors

М. А. Аzhigirova
Гематологический научный центр РАМН
Russian Federation


T. L. Dereza
Гематологический научный центр РАМН
Russian Federation


I. S. Кryazhevskikh
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


N. V. Sergeeva
Гематологический научный центр РАМН
Russian Federation


V. I. Shvets
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


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For citation:


Аzhigirova М.А., Dereza T.L., Кryazhevskikh I.S., Sergeeva N.V., Shvets V.I. Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III. Fine Chemical Technologies. 2010;5(2):64-68. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)