DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL

In the present study, a rapid, sensitive, and selective method for determination of several synthetic analogues of gonadotropin-releasing hormone in human urine by solid-phase extraction and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed. Various parameters affecting sample preparation, LC separation, and MS/MS detection were investigated, and optimized conditions were identified. The UPLC-MS/MS system was equipped with an electrospray ion source operating in positive ion mode with selected reaction monitoring. Leuprolide-13С6 was used as internal standard for analytes quantitative assessment. The proposed method was validated considering the parameters specificity, linearity (0.1-10 ng/ml), recovery (52-98%), limit of detection (0.1 ng/ml), matrix effects and stability.

gonadotropin-releasing hormone, gonadotropin-releasing hormone agonists, solidphase egonadotropin-releasing hormon, gonadotropin-releasing hormon agonists, solid-phase extraction, ultra-performance liquid chromatography coupled to tandem mass spectrometry, UPLCMS/MS, ultra-performance liquid chromatography coupled to high resolution mass spectrometry, UPLC-HRMS, doping control

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