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Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers

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Abstract

An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for each PANAM monomer and estimation of the relative content (abundance ratio) of main and minor diastereomers by means of reverse phase HPLC and 1H-NMR spectroscopy.

About the Authors

Yu. G. Kirillova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


A. V. Ryakin
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


D. I. Prokhorov
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


M. A. Lyanov
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


O. V. Esipova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


S. V. Eremin
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


E. E. Zvonkova
M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571
Russian Federation


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For citation:


Kirillova Yu.G., Ryakin A.V., Prokhorov D.I., Lyanov M.A., Esipova O.V., Eremin S.V., Zvonkova E.E. Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers. Fine Chemical Technologies. 2012;7(2):70-74. (In Russ.)

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ISSN 2410-6593 (Print)
ISSN 2686-7575 (Online)