OPTIMIZATION OF SOLID-PHASE SYNTHESIS OF POLYAMIDE NUCLEIC ACID MIMETICS WITH APPLICATION OF MALDI-TOF MASS-SPECTROMETRY

The solid-phase synthesis of a tetrameric model sequence of polyamide nucleic acid mimetics (PANAM) incorporating terminal chiral and charged unit was performed. As a result during the synthesis and cleavage from the resin the tendency of PANAM with the N-terminal negatively charged monomer to cyclization with simultaneous N-acyl transfer of carboxyethyl base residue was found. Application of MALDI-TOF mass - spectrometry allowed us to find suitable conditions for obtaining PANAM oligomers without the significant amount of by-products.

полиамидные миметики нуклеиновых кислот, пептидно-нуклеиновые кислоты (ПНК), твердофазный синтез, MALDI-TOF-масс-спектрометрия, polyamide nucleic acid mimetics (PANAM), peptide nucleic acids (PNA), solid phase synthesis, MALDI-TOF mass-spectrometry

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