SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY
https://doi.org/10.32362/2410-6593-2017-12-4-75-84
Abstract
This paper presents the results of studies of seed copolymerization of glycidyl methacrylate with ethylene glycol poly(glycidyl methacrylate) on seed particles in order to obtain partially crosslinked polymer microspheres with a diameter of about 3.5 microns for use in immunochemical reactions, as carriers of bioligands. The physico-chemical properties of polymeric microspheres obtained under different conditions were studied by the following methods: the diameters of the particles - by electron scanning and light microscopy; the wetting angle - bу the method of "lying droplets"; the sedimentation velocity - by the macromethod in a capillary tube by the movement of the (polymer slurry)/water phase boundary (buffer solution); the zeta-potential - by dynamic light scattering. Having studied the physical-chemical properties of all polymer slurries we concluded that the optimal particles to create on the basis of their diagnostic test systems with high sensitivity working on the principle of latex agglutination reaction are poly(glycidyl methacrylate) particles with equal percentage of glycidyl methacrylate and ethylene glycol dimethacrylate aminated with hexamethylendiamine in the environment of n-propanol, because they have a sufficient number of amino groups and do not lose their sedimentation properties after all stages of the synthesis.
About the Authors
A. V. Bakhtina
Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Moscow 119571, Russia
Russian Federation
Moscow 119571, Russia
A. A. Sivaev
Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Moscow 119571, Russia
Russian Federation
Moscow 119571, Russia
S. M. Levachev
M.V. Lomonosov Moscow State University
Russian Federation
Moscow 119899, Russia
Russian Federation
Moscow 119899, Russia
S. A. Gusev
Federal Scientific-Clinical Center of Physico-Chemical Medicine FMBA of Russia
Russian Federation
Moscow 119435, Russia
Russian Federation
Moscow 119435, Russia
N. A. Lobanova
Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Moscow 119571, Russia
Russian Federation
Moscow 119571, Russia
M. A. Lazov
Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Moscow 119571, Russia
Russian Federation
Moscow 119571, Russia
I. A. Gritskova
Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Moscow 119571, Russia
Russian Federation
Moscow 119571, Russia
References
1. Cao K., Yu J., Li B.-G., Li B.-F., Pan Z.-R. Micron-size uniform poly(methyl methacrylate) particles by dispersion polymerization in polar media: 1. Particle size and particle size distribution //Chem. Eng. J. 2000. V. 78. Iss. 2-3. P. 211–215.
2. Kim J.-W., Suh K.-D. Highly monodisperse crosslinked polystyrene microparticles by dispersion polymerization // Colloid and Polymer Science. 1998. V. 276. Iss. 10. P. 870–878.
3. Volkova E.V., Gritskova I.A., Gusev S.A., Lukashevich A.D., Gusev A.A., Levshenko E.N., Zlydnev L.A., Sochilina K.O.. Development of polymeric microspheres for immunofluorescence analysis // Biotekhnologiya (Biotechnology). 2012. № 4. P. 74–77. (in Russ.).
4. Volkova E.V., Lukashevich A.D., Levichev I.S., Levachev S.M., Gusev S.A., Gritskova I.A.. Selection of polymeric microspheres for carrying out the latex agglutination reaction in the spot format // Vestnik MITHT (Fine Chemical Technologies). 2013. T. 8. № 6. P. 68–72. (in Russ.).
5. Prokopov N.I., Gritskova I.A., Cherkasov V.R., Chalykh A.E. Synthesis of monodisperse functional polymeric microspheres for immunodiagnostics research // Uspekhi khimii (Russian Chem. Rev.). 1996/ V. 65. № 2. P. 178–192. (in Russ.).
6. Sandzhieva A.V., Bakhtin V.A., Sivaev A.A., Batyreva L.Y., Gusev S.A., Gritskova I.A. Ways to improve specificity of the latex agglutination reaction // Tonkie khimicheskie tekhnologii (Fine Chemical Technologies). 2016. V. 11. № 2. P. 17–22. (in Russ.).
7. Paine, A.J. Dispersion polymerization of styrene in polar solvents. A simple mechanistic model to predict particle size // Macromolecules. 1990. V. 23. № 12. P. 3109–3117.
8. Paine A. J., Luymes W., McNulty J. Dispersion polymerization of styrene in polar solvents. 6. Influence of reaction parameters on particle size and molecular weight in poly(N-vinylpyrrolidone)-stabilized reactions // Macromolecules. 1990. V. 23. 12. P. 3104–3109.
9. Kawaguchi S., Winnik M.A., Ito K. Dispersion copolymerization of N-butylmethacrylat with poly(ethylene oxide) macromolecules in methanolwater – Comparison of experiment with theory // Macromolecules. 1995. V. 28. № 4. P. 1159–1166.
10. Tseng C.M., Lu Y.Y., El Aasser M.S., Vanderhoff J.W. Uniform polymer particles by dispersion polymerization in alcohol // J. Polym. Sci. Polym. Chem. 1986. V. 24. № 11. P. 2995–3007.
Review
For citations:
Bakhtina A.V., Sivaev A.A., Levachev S.M., Gusev S.A., Lobanova N.A., Lazov M.A., Gritskova I.A. SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY. Fine Chemical Technologies. 2017;12(4):75-84. (In Russ.) https://doi.org/10.32362/2410-6593-2017-12-4-75-84
Views: 394
Email this article 
