chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).10.32362/2410-6593-2022-17-2-131-139chemicallytech-1821Research ArticleCHEMISTRY AND TECHNOLOGY OF MEDICINAL COMPOUNDS AND BIOLOGICALLY ACTIVE SUBSTANCESХИМИЯ И ТЕХНОЛОГИЯ ЛЕКАРСТВЕННЫХ ПРЕПАРАТОВ И БИОЛОГИЧЕСКИ АКТИВНЫХ СОЕДИНЕНИЙScreening medicinal plant extracts for xanthine oxidase inhibitory activityСкрининг экстрактов лекарственных растений на ингибирующую активность ксантиноксидазыhttps://orcid.org/0000-0001-7919-4028ХаА. К.HaA. C.

Ань К. Ха, PhD, к.м.н., Химико-технологический факультет

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин; Линь Чунг Уорд, Район Тхёк, г. Хошимин

Anh C. Ha, PhD., Doctor of Medicinal Chemistry, Faculty of Chemical Engineering

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City; Linh Trung Ward, Thu Duc District, Ho Chi Minh City

hcanh@hcmut.edu.vnhttps://orcid.org/0000-0002-2820-3913НгуенЧ. Д.П.NguyenCh. D.P.

Чин Д.П. Нгуен, аспирант, Химико-технологический факультет

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин; Линь Чунг Уорд, Район Тхёк, г. Хошимин

Chinh D.P. Nguyen, BEng., Postgraduate Student, Faculty of Chemical Engineering

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City; Linh Trung Ward, Thu Duc District, Ho Chi Minh City

npdchinh.sdh19@hcmut.edu.vnhttps://orcid.org/0000-0002-7956-1067ЛеТ. М.LeT. M.

Тан М. Ле, аспирант, Химико-технологический факультет

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин; Линь Чунг Уорд, Район Тхёк, г. Хошимин

Tan M. Le, MSc., Master of Chemical Engineering, Faculty of Chemical Engineering

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City; Linh Trung Ward, Thu Duc District, Ho Chi Minh City

lmtan.sdh20@hcmut.edu.vnТехнологический университет Хошимина; Вьетнамский национальный университетВьетнамHo Chi Minh City University of Technology (HCMUT); Vietnam National University Ho Chi Minh City (VNU-HCM)Viet Nam202231052022172131139Copyright © Ha A.C., Nguyen C.D., Le T.M., 20222022Ха А.К., Нгуен Ч.Д., Ле Т.М.Ha A.C., Nguyen C.D., Le T.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/1821Objectives

Objectives. The study aimed to test the ethanol extracts of ten medicinal plants for xanthine oxidase inhibitory activity.

Methods

Methods. The degree of xanthine oxidase inhibitory activity was determined by measuring the absorbance spectrophotometrically at 290 nm, which is associated with uric acid formation. The selected medicinal plants included Piper lolot C.DC. (Piperaceae), Pandanus amaryllifolius R.(Pandanaceae), Brassica juncea L. (Brassicaceae), Piper betle L. (Piperaceae), Perilla frutescens L. (Lamiaceae), Anacardium occidentale L. (Anacardiaceae), Polygonum barbatum L. (Polygonaceae), Artocarpus Altilis P. (Moraceae), Vitex negundo L. (Verbenaceae), Annona squamosal L. (Annonaceae), which were selected based on folk medicine.

Results

Results. The results showed that the Piper betle L. has a strong ability to inhibit xanthine oxidase with an IC50 value of up to 1.18 μg/mL, compared to allopurinol 1.57 μg/mL. Different parts of Piper betle L. were compared and the leaves of Piper betle L. showed the best value for xanthine oxidase inhibitory and antioxidant activity.

Conclusions

Conclusions. Piper betle L. showed the best potential for inhibition of xanthine oxidase among ten medicinal plants. Piper betle L. leaf extract showed strong xanthine oxidase inhibitory and antioxidant activity, compared to the whole plant, and the stem extract, which promises to be applied in the treatment of gout.

Цели

Цели. Исследование было направлено на проверку этанольных экстрактов десяти лекарственных растений на ингибиторную активность ксантиноксид азы.

Методы

Методы. Степень ингибирующей активности ксантиноксидазы определяли путем спектрофотометрического измерения поглощения при 290 нм, вызываемого образованием мочевой кислоты. В состав отобранных лекарственных растений вошли перец-лолот (Piperaceae), пандан (Pandanaceae), горчица сарептская (Brassicaceae), бетель (Piperaceae), перилла обыкновенная (Lamiaceae), кешью (Anacardiaceae), конопля (Polygonaceae), хлебное дерево (Moraceae), прутняк китайский (Verbenaceae), сахарное яблоко (Annonaceae), отобранные на основе их применения в народ ной медицине.

Результаты

Результаты. Результаты показали, что бетель обладает сильной способностью ингибировать ксантиноксидазу со значением IC50 до 1.18 мкг/мл по сравнению с аллопуринолом 1.57 мкг/мл. Были проведены сравнения различных частей бетеля, и листья бетеля показали наилучшие показатели ингибирования ксантиноксидазы и антиоксидантной активности.

Выводы

Выводы. Бетель показал лучший потенциал ингибирования ксантиноксидазы среди десяти лекарственных растений. Экстракт листьев бетеля показал сильное подавление ксантиноксидазы и антиоксидантную активность по сравнению с целым растением и экстрактом стебля, которые применяются при лечении подагры.

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The authors declare that there are no conflicts of interest present.