chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).10.32362/2410-6593-2021-16-3-232-240chemicallytech-1711Research ArticleCHEMISTRY AND TECHNOLOGY OF MEDICINAL COMPOUNDS AND BIOLOGICALLY ACTIVE SUBSTANCESХИМИЯ И ТЕХНОЛОГИЯ ЛЕКАРСТВЕННЫХ ПРЕПАРАТОВ И БИОЛОГИЧЕСКИ АКТИВНЫХ СОЕДИНЕНИЙCombination of Phyllanthus amarus Schum. & Thonn. and Gymnema sylvestre R. Br. for treatment of diabetes and its long-term complicationsКомбинация Phyllanthus amarus Schum. & Thonn. и Gymnema sylvestre R. Br. для лечения диабета и его долгосрочных осложненийhttps://orcid.org/0000-0002-7956-1067ЛеТ. М.LeT. M.

Тан М. Ле, аспирант

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин

Линь Чунг Уорд, Район Тхёк, г. Хошимин

Tan M. Le, BEng., Postgraduate Student

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 

lmtan.sdh20@hcmut.edu.vnhttps://orcid.org/0000-0002-2820-3913НгуенЧ.Д. П.NguyenCh. D.P.

Чин Д.П. Нгуен, аспирант

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин

Линь Чунг Уорд, Район Тхёк, г. Хошимин

Chinh D.P. Nguyen, BEng., Postgraduate Student

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 

npdchinh.sdh19@hcmut.edu.vnhttps://orcid.org/0000-0001-7919-4028ХаА. К.HaA. C.

Ань К. Ха, PhD, к.х.н.

268 Ли Тхыонг Кьет ул., Район 10, г. Хошимин

Линь Чунг Уорд, Район Тхёк, г. Хошимин

Anh C. Ha,PhD, Dr. of Chemical Engineering

268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City

Linh Trung Ward, Thu Duc District, Ho Chi Minh City 

hcanh@hcmut.edu.vnХимико-технологический факультет, Технологический университет Хошимина; Вьетнамский национальный университетВьетнамFaculty of Chemical Engineering, Ho Chi Minh City University of Technology; Vietnam National University Ho Chi Minh CityViet Nam202122072021163232240Copyright © Le T.M., Nguyen C.D., Ha A.C., 20212021Ле Т.М., Нгуен Ч.П., Ха А.К.Le T.M., Nguyen C.D., Ha A.C.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/1711

Objectives. The amount of patients with diabetes is increasing, and such patients experience several long-term complications. Therefore, finding a method to treat the disease and its complications is an urgent issue worldwide. In Vietnam, Phyllanthus amarus Schum. & Thonn. (PA) and Gymnema sylvestre R. Br. (GS) are common herbs used in traditional therapy including diabetes treatment. This study aimed to combine PA and GS to extend their bioactivities in antidiabetes, antioxidant, and anti-inflammatory treatments.Methods. Here, PA and GS powders were mixed at different ratios for extraction. Ethanolic extract was used to detect bioactive compounds, bioactivities, and appropriate ratios of the mixtures.Results. The optimal ratio for the PA and GS combination was 2 : 1 (g/g). The ethanolic extraction of the 2 : 1 sample at 50°C over two hours with a solid/liquid ratio of 1 : 10 achieved a high yield of 14.37%. This sample exhibited good α-glucosidase inhibition activity with a half-maximal inhibitory concentration (IC50) of 9.74 µg/mL, antioxidant activity with an IC50 of 29.87 µg/mL, and anti-inflammatory activity with an IC15 of 400 µg/mL.Conclusions. The study confirmed that combining PA and GS can have high α-glucosidase inhibition as well as antioxidant and anti-inflammatory effects.

Цели. Количество пациентов с диабетом растет, у них часто возникают долгосрочные осложнения, поэтому поиск методов лечения этого заболевания и коррекции его осложнений является важным для всего мирового медицинского сообщества. Phyllanthus amarus Schum. & Thonn. (PA) и Gymnema sylvestre R. Br. (GS) – распространенные во Вьетнаме лекарственные растения, используемые в традиционной медицине, включая лечение диабета. Цель данного исследования – скомбинировать PA и GS, чтобы расширить их биологическую активность и усилить антидиабетический, антиоксидантный и противовоспалительный эффект.Методы. Порошки листьев PA и GS смешивали в различных соотношениях и экстрагировали 95% этанолом. Полученные этанольные экстракты использовались для определения биологически активных соединений, биологической активности и оптимального соотношения компонентов смеси.Результаты. Оптимальное соотношение PA и GS, определенное в исследовании, равно 2 : 1 (г/г). Экстракция 95% этанолом данного образца (2 : 1) при 50 °C в течение двух часов при соотношении сырье/эстрагент 1 : 10 позволила получить высокий выход экстрактивных веществ, равный 14.37%. Этот образец продемонстрировал хорошую активность ингибирования α-глюкозидазы с половинной максимальной ингибирующей концентрацией (IC50) 9.74 мкг/мл, антиоксидантную активность с IC50 29.87 мкг/мл и противовоспалительную активность с IC15 400 мкг/мл.Выводы. Исследование подтвердило, что сочетание PA и GS может значительно ингибировать α-глюкозидазу, а также обладает антиоксидантным и противовоспалительным эффектами.

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The authors declare that there are no conflicts of interest present.