chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).10.32362/2410-6593-2018-13-2-31-39chemicallytech-139Research ArticleCHEMISTRY AND TECHNOLOGY OF MEDICINAL COMPOUNDS AND BIOLOGICALLY ACTIVE SUBSTANCESХИМИЯ И ТЕХНОЛОГИЯ ЛЕКАРСТВЕННЫХ ПРЕПАРАТОВ И БИОЛОГИЧЕСКИ АКТИВНЫХ СОЕДИНЕНИЙINFLUENCE OF POLYMYXIN B ON THE FORMATION OF BIOFILMS BY BACTERIUM Methylophilus quaylei ON POLYPROPYLENE AND TEFLONВЛИЯНИЕ ПОЛИМИКСИНА В НА ФОРМИРОВАНИЕ БИОПЛЕНКИ БАКТЕРИЕЙ Methylophilus quaylei НА ПОЛИПРОПИЛЕНЕ И ТЕФЛОНЕМохамедА. М.MohamedA. M.

аспирантка кафедры биотехнологии и промышленной фармации

119571, Россия, Москва, проспект Вернадского, д. 86

Postgraduate Student of the Chair of Biotechnology and Industrial Pharmacy

86, Vernadskogo Pr., Moscow, 119571, Russia

noemail@neicon.ruАмзаеваД. Н.AmzaevaD. N.

студентка кафедры биотехнологии и промышленной фармации

119571, Россия, Москва, проспект Вернадского, д. 86

Student of the Chair of Biotechnology and Industrial Pharmacy

86, Vernadskogo Pr., Moscow, 119571, Russia

noemail@neicon.ruПшеничниковаА. Б.PshenichnikovaA. B.

доцент, кандидат химических наук, доцент кафедры биотехнологии и промышленной фармации

119571, Россия, Москва, проспект Вернадского, д. 86

Docent, Ph.D. (Chemistry), Associate Professor of the Chair of Biotechnology and Industrial Pharmacy

86, Vernadskogo Pr., Moscow, 119571, Russia

a_pshenichnikova@mail.ruШвецВ. И.ShvetsV. I.

академик РАН, профессор, доктор химических наук, профессор кафедры биотехнологии и промышленной фармации

119571, Россия, Москва, проспект Вернадского, 86

Academician of the Russian Academy of Sciences, D.Sc. (Chemistry), Professor of the Chair of Biotechnology and Industrial Pharmacy

86, Vernadskogo Pr., Moscow, 119571, Russia

noemail@neicon.ruМосковский технологический университет (Институт тонких химических технологий им. М.В. Ломоносова)РоссияMoscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Russian Federation2018280420181323139Copyright © Mohamed A.M., Amzaeva D.N., Pshenichnikova A.B., Shvets V.I., 20182018Мохамед А.М., Амзаева Д.Н., Пшеничникова А.Б., Швец В.И.Mohamed A.M., Amzaeva D.N., Pshenichnikova A.B., Shvets V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/139

Here we examined the influence of polymyxin B, a lipopeptide antibiotic, on planktonic growth and biofilm of streptomycin-sensitive and streptomycin-resistant isogenic strains of bacteria Methylophilus quaylei on polypropylene and Teflon. Biofilm formation was quantified using crystal violet staining, determined by colony-forming unit and examined by light microscopy. It was found that of bacterial surface hydrophobicity affects the biofilm formation: biofilm formation of Methylophilus quaylei MT strain was better on such hydrophobic materials as Teflon and polypropylene. The minimum inhibitory concentration of polymyxin B for the used strains in planktonic and biofilm form was 1 μg/ml. The sublethal Polymyxin B in the concentration of 0.01 μg/ml stimulates biofilm formation and exhibits antibiofilm properties at the concentration of 0.10 μg/ml. Synergistic effect of polymyxin B and streptomycin on streptomycin-resistant strain M. quaylei SM was found.

Исследовано влияние липопептидного антибиотика полимиксина В на рост чувствительного и резистентного к стрептомицину изогенных штаммов метилотрофной бактерии Methylophilus quaylei в планктоне и биопленке на полипропилене и тефлоне. Интенсивность образования биопленок определяли по окрашиванию кристаллическим фиолетовым, колониеобразующей способности, а также методом световой микроскопии. Обнаружено влияние гидрофобности клеточной поверхности бактерий на способность образовывать биопленки. На пластинах из тефлона и полипропилена более интенсивную биопленку образует штамм Methylophilus quaylei MT с гидрофобной поверхностью. Минимальная ингибирующая концентрация полимиксина В для изученных штаммов и в планктонной, и в биопленочной форме составила 1 мкг/мл. Полимиксин В в сублетальной концентрации 0.01 мкг/мл стимулирует образование биопленок, а в концентрации 0.10 мкг/мл проявляет антибиопленочные свойства. Обнаружено синергидное действие полимиксина В и стрептомицина на резистентный к стрептомицину штамм M. quaylei SM.

бактериальные биопленкиполимиксинрезистентность к антибиотикамметилотрофные бактерииbacterial biofilmspolymyxinresistance to antibioticsmethylotrophic bacteriaReferencesCosterton J.W. Overview of microbial biofilms // J. Ind. Microbiol. 1995. V. 15. P. 137-140.Costerton J.W. Overview of microbial biofilms // J. Ind. Microbiol. 1995. V. 15. P. 137–140.Flemming H.C., Wingender J., Szewzyk U., Steinberg P., Rice S.A., Kjelleberg S. Biofilms: An emergent form of bacterial life // Nat. Rev. Microbiol. 2016. V. 14. № 9. P. 563-575.Flemming H.C., Wingender J., Szewzyk U., Steinberg P., Rice S.A., Kjelleberg S. Biofilms: An emergent form of bacterial life // Nat. Rev. Microbiol. 2016. V. 14. № 9. P. 563–575.Flemming H.C., Wingender J. The biofilm matrix // Nat. Rev. Microbiol. 2010. V. 8. Р. 623-633.Flemming H.C., Wingender J. The biofilm matrix // Nat. Rev. Microbiol. 2010. V. 8. Р. 623–633.Zhurina M.V., Gannesen A.V., Zdorovenko E.L., Plakunov V.K. Composition and functions of the extracellular polymer matrix of bacterial biofilms // Microbiology. 2014. V. 83. № 6. P. 713-722.Zhurina M.V., Gannesen A.V., Zdorovenko E.L., Plakunov V.K. Composition and functions of the extracellular polymer matrix of bacterial biofilms // Microbiology. 2014. V. 83. № 6. P. 713–722.Jiao Y., Cody G.D., Harding A.K., Wilmes P., Schrenk M., Wheeler K.E., Banfield J.F., Thelen M.P. Characterization of extracellular polymeric substances from acidophilic microbial biofilms // Appl. Environ. Microbiol. 2010. V. 76. № 9. P. 2916-2922.Jiao Y., Cody G.D., Harding A.K., Wilmes P., Schrenk M., Wheeler K.E., Banfield J.F., Thelen M.P. Characterization of extracellular polymeric substances from acidophilic microbial biofilms // Appl. Environ. Microbiol. 2010. V. 76. № 9. P. 2916–2922.Stewart P.S., Costerton J.W. Antibiotic resistance of bacteria in biofilms // Lancet. 2001. V. 358. P. 135-138.Stewart P.S., Costerton J.W. Antibiotic resistance of bacteria in biofilms // Lancet. 2001. V. 358. P. 135–138.Плакунов В.К., Мартьянов С.В., Тетенева Н.А., Журина М.В. Управление формированием микробных биопленок: анти- и пробиопленочные агенты // Микробиология. 2017. Т. 86. № 4. С. 402-420.Plakunov V.K., Martyanov S.V., Teteneva N.A., Zhurina M.V. Management of the formation of microbial biofilms: Anti- and probiofilm agents // Mikrobiologiya (Microbiology). 2017. V. 86. № 4. P. 402–420. (in Russ.).Costerton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: A common cause of persistent infections // Science. 1999. V. 284. Issue 5418. P. 1318-1322.Costerton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: A common cause of persistent infections // Science. 1999. V. 284. Issue 5418. P. 1318–1322.Rabin N., Zheng Y., Opoku-Temeng C., Du Y., Bonsu E., Sintim H.O. Agents that inhibit bacterial biofilm formation // Future Med. Chem. 2015. V. 7. № 5. P. 647-671.Rabin N., Zheng Y., Opoku-Temeng C., Du Y., Bonsu E., Sintim H.O. Agents that inhibit bacterial biofilm formation // Future Med. Chem. 2015. V. 7. № 5. P. 647–671.Petrova O.E., Sauer K. Escaping the biofilm in more than one way: Desorption, detachment or dispersion // Curr. Opin. Microbiol. 2016. V. 30. Р. 67-78.Petrova O.E., Sauer K. Escaping the biofilm in more than one way: Desorption, detachment or dispersion // Curr. Opin. Microbiol. 2016. V. 30. Р. 67–78.Estrela A.B., Abraham W.R. Combining biofilmcompounds and controlling antibiotics as a promising new way to control biofilm infections // Pharmaceuticals. 2010. V. 3. Р. 1374-1393.Estrela A.B., Abraham W.R. Combining biofilmcompounds and controlling antibiotics as a promising new way to control biofilm infections // Pharmaceuticals. 2010. V. 3. Р. 1374–1393.Koo H., Allan R.N., Howlin R.P., Stoodley P., Stoodley L.H. Targeting microbial biofilms: Current and prospective therapeutic strategies // Nature Rev. Microbiol. 2017. V. 15. № 12. P. 740-755.Koo H., Allan R.N., Howlin R.P., Stoodley P., Stoodley L.H. Targeting microbial biofilms: Current and prospective therapeutic strategies // Nature Rev. Microbiol. 2017. V. 15. № 12. P. 740–755.Стрелкова Е.А., Журина М.В., Плакунов В.К., Беляев С.С. Стимуляция антибиотиками процесса формирования бактериальных биопленок // Микробиология. 2012. Т. 81. № 2. С. 282-285.Strelkova E.A., Zhurina M.V., Plakunov V.K., Belyaev S.S. Stimulation of bacterial biofilms formation with antibiotics // Mikrobiologiya (Microbiology). 2012. V. 81. № 2. P. 282–285. (in Russ.).Wu S., Li X., Gunawardana M., Maguire K., Guerrero-Given D., Schaudinn C., Wang C., Baum M.M., Webster P. Beta-lactam antibiotics stimulate biofilm formation in non-typeable haemophilus influenzae by up-regulating carbohydrate metabolism // PLoS ONE. 2014. V. 9. Iss. 7. e99204.Wu S., Li X., Gunawardana M., Maguire K., Guerrero-Given D., Schaudinn C., Wang C., Baum M.M., Webster P. Beta-lactam antibiotics stimulate biofilm formation in non-typeable haemophilus influenzae by up-regulating carbohydrate metabolism // PLoS ONE.Kaplan J.B. Antibiotic-induced biofilm formation // Int. J. Artif. Organs. 2011. V. 34. № 9. P.737-751.V. 9. Iss. 7. e99204.Velkov T., Thompson P. E., Nation R. L., Li J. Structure-activity relationships of polymyxin antibiotics // J. Med. Chem. 2010. V. 53. № 5. P. 1898-1916.Kaplan J.B. Antibiotic-induced biofilm formation // Int. J. Artif. Organs. 2011. V. 34. № 9. P.737–751.Rabanal F., Cajal Y. Recent advances and perspectives in the design and development of polymyxins // Nat. Prod. Rep. 2017. V. 6. № 7. P. 886-908.Velkov T., Thompson P. E., Nation R. L., Li J. Structure-activity relationships of polymyxin antibiotics // J. Med. Chem. 2010. V. 53. № 5. P. 1898–1916.Velkov T., Roberts K.D., Nation R.L., Thompson P.E., Li J. Pharmacology of polymyxins: New insights into an ‘old’ class of antibiotics // Future Microbiol. 2013. V. 8. № 6. Р. 711-724.Rabanal F., Cajal Y. Recent advances and perspectives in the design and development of polymyxins // Nat. Prod. Rep. 2017. V. 6. № 7. P. 886–908.Kaye K.S., Pogue J.M., Tran T.B., Nation R.L., Li J. Agents of last resort: Polymyxin resistance // Infect. Dis. Clin. North. Am. 2016. V. 30. № 2. P. 391-414.Velkov T., Roberts K.D., Nation R.L., Thompson P.E., Li J. Pharmacology of polymyxins: New insights into an ‘old’ class of antibiotics // Future Microbiol. 2013. V. 8. № 6. Р. 711–724.Srinivas P., Rivard K. Polymyxin resistance in Gram-negative pathogens // Curr. Infect. Dis. Rep. 2017. V. 19. № 11. P. 38.Kaye K.S., Pogue J.M., Tran T.B., Nation R.L., Li J. Agents of last resort: Polymyxin resistance // Infect. Dis. Clin. North. Am. 2016. V. 30. № 2. P. 391–414.Jeannot K., Bolard A., Plesiat P. Resistance to polymyxins in Gram-negative organisms // Int. J. Antimicrob. Agents. 2017. V. 49. № 5. P. 526-535.Srinivas P., Rivard K. Polymyxin resistance in Gram-negative pathogens // Curr. Infect. Dis. Rep. 2017. V. 19. № 11. P. 38.Brown P., Dawson M.J. Development of new polymyxin derivatives for multi-drug resistant Gramnegative infections // J. Antibiot. (Tokyo). 2017. V. 70. № 4. P. 386-394.Jeannot K., Bolard A., Plésiat P. Resistance to polymyxins in Gram-negative organisms // Int. J. Antimicrob. Agents. 2017. V. 49. № 5. P. 526–535.Pye C.C., Yu A.A., Weese J.S. Evaluation of biofilm production by Pseudomonas aeruginosa from canine ears and the impact of on antimicrobial susceptibility in vitro // Vet. Dermatol. 2013. V. 24. № 4. P. 446-449. e98-9.Brown P., Dawson M.J. Development of new polymyxin derivatives for multi-drug resistant Gramnegative infections // J. Antibiot. (Tokyo). 2017. V. 70. № 4. P. 386–394.Doronina N., Ivanova E., Trotsenko Y., Pshenichnikova A., Kalinina E., Shvets V. Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium // System. Appl. Microbiol. 2005. V. 28. P. 303-309.Pye C.C., Yu A.A., Weese J.S. Evaluation of biofilm production by Pseudomonas aeruginosa from canine ears and the impact of on antimicrobial susceptibility in vitro // Vet. Dermatol. 2013. V. 24. № 4. P. 446–449. e98-9.Пшеничникова А.Б., Гаврилова Е.С., Швец В.И. Влияние физико-химических свойств клеточной поверхности грамотрицательных бактерий на резистентность к стрептомицину // Вестник МИТХТ. 2011. Т. 6. № 2. С. 43-50.Doronina N., Ivanova E., Trotsenko Y., Pshenichnikova A., Kalinina E., Shvets V. Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium // System. Appl. Microbiol. 2005. V. 28. P. 303–309.Отман С.А.М., Пшеничникова А.Б., Швец В.И. Влияние экзогенных жирных кислот на рост и продукцию экзополисахарида облигатной метилотрофной бактерии Methylophilus quaylei // Прикл. биохимия и микробиология. 2012. Т. 48. № 2. С. 226-231.Pshenichnikova A.B., Gavrilova E.S., Shvets V.I. Influence of physico-chemical properties of the gram-negative bacteria cell surface on the resistance to streptomycin // Vestnik MITHT (Fine Chemical Technlolgies). 2011. V. 6. № 2. P. 43–50. (in Russ.).ОФС.1.7.2.0008.15. Определение концентрации микробных клеток. Государственная фармакопея Российской Федерации. XIII издание. Т.II. Москва, 2015. OFS.1.7.2.0008.15.Otman S.A.M., Pshenichnikova A.B., Shvets V.I. Influence of exogenous fatty acids on the growth and production of the exopolysaccharide of the obligate methylotrophic bacterium Methylophilus quaylei // Prikladnaya biokhimiya i mikrobiologiya (Russian Journal of Applied Biochemistry and Microbiology). 2012. V. 48. № 2. P. 226–231. (in Russ.).Rosenberg M., Gutniek D., Rosenberg E. Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity // FEMS Microbiol. Lett. 1980. V. 9. P. 29-33.OFS.1.7.2.0008.15. Determination of the concentration of microbial cells. State Pharmacopoeia of the Russian Federation. XIII edition. V. II. Moscow, 2015.Anesti V., McDonald I.R., Ramaswamy M., Wade W.G., Kelly D.P., Wood A.P. Isolation and molecular detection of methylotrophic bacteria occurring in the human mouth // Environmen. Microbiol. 2005. V. 7. № 8. P. 1227-1238.Rosenberg M., Gutniek D., Rosenberg E. Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity // FEMS Microbiol. Lett. 1980. V. 9. P. 29–33.Gogleva A.A., Kaparullina E.N., Doronina N.V., Trotsenko Y.A. Methylophilus flavus sp. nov., and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants // Int. J. Syst. Evol. Microbiol. 2010. V. 60. P. 2623-2628.Anesti V., McDonald I.R., Ramaswamy M., Wade W.G., Kelly D.P., Wood A.P. Isolation and molecular detection of methylotrophic bacteria occurring in the human mouth // Environmen. Microbiol. 2005. V. 7. № 8. P. 1227–1238.Cerca N., Pier G. B., Vilanova M., Oliveira R., Azeredo J. Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis // Res. Microbiology. 2005. V. 156. № 4. P. 506-514.Gogleva A.A., Kaparullina E.N., Doronina N.V., Trotsenko Y.A. Methylophilus flavus sp. nov., and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants // Int. J. Syst. Evol. Microbiol. 2010. V. 60. P. 2623–2628.Oliveira R., Azeredo J., Fonseca A.P. The role of hydrophobicity in bacterial adhesion // Hydrophobicity and Adhesion. 2001. P. 11-22.Cerca N., Pier G. B., Vilanova M., Oliveira R., Azeredo J. Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis // Res. Microbiology. 2005. V. 156. № 4. P. 506–514.Maitz M.F. Applications of synthetic polymers in clinical medicine // Biosurface and Biotribology. 2015. V. 1. Р. 161-176.Oliveira R., Azeredo J., Fonseca A.P. The role of hydrophobicity in bacterial adhesion // Hydrophobicity and Adhesion. 2001. P. 11–22.Lenhard J.R., Nation R.L., Tsuji B.T. Synergistic combinations of polymyxins // Int. J. Antimicrob. Agents. 2016. V. 48. № 6. Р. 607-613.Maitz M.F. Applications of synthetic polymers in clinical medicine // Biosurface and Biotribology. 2015. V. 1. Р. 161–176.Lenhard J.R., Nation R.L., Tsuji B.T. Synergistic combinations of polymyxins // Int. J. Antimicrob. Agents. 2016. V. 48. № 6. Р. 607–613.Lenhard J.R., Nation R.L., Tsuji B.T. Synergistic combinations of polymyxins // Int. J. Antimicrob. Agents. 2016. V. 48. № 6. Р. 607–613.

The authors declare that there are no conflicts of interest present.