chemicallytechFine Chemical TechnologiesТонкие химические технологии2410-65932686-7575MIREA – Russian Technological University (RTU MIREA).chemicallytech-1191Research ArticleCHEMISTRY AND TECHNOLOGY OF MEDICINAL COMPOUNDS AND BIOLOGICALLY ACTIVE SUBSTANCESХИМИЯ И ТЕХНОЛОГИЯ ЛЕКАРСТВЕННЫХ ПРЕПАРАТОВ И БИОЛОГИЧЕСКИ АКТИВНЫХ СОЕДИНЕНИЙTest system creation for analysis of template switching effect during a reverse transcription in vitroРазработка тест-системы для анализа эффекта «смены матрицы» при обратной транскрипции in vitroКомковА. Ю.KomkovA. Y.
аспирант, инженер- исследователь
alexandrkomkov@mail.ruЛебедевЮ. Б.LebedevY. B.
заведующий лабораторией
noemail@neicon.ruШвецВ. И.ShvetsV. I.
кафедра Биотехнологии и бионанотехнологии, заведующий кафедрой
noemail@neicon.ruИнститут биоорганической химии им. академиков
М.М. Шемякина и Ю.А. Овчинникова РАНRussian FederationМИТХТ им. М.В. Ломоносова, 119571, Москва, пр-т Вернадского, д. 86РоссияM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Russian Federation201128122011667783Copyright © Komkov A.Y., Lebedev Y.B., Shvets V.I., 20112011Комков А.Ю., Лебедев Ю.Б., Швец В.И.Komkov A.Y., Lebedev Y.B., Shvets V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.finechem-mirea.ru/jour/article/view/1191Alu-повторетроэлементобратная транскриптазаэффект «смены матрицы»Alu repeatretroelementreverse transcriptasetemplate switching effectReferencesAmariglio N., Rechavi G. A-to-I RNA editing: А new regulatory mechanism of global gene expression // Blood Cells Mol. Dis. 2007. V. 39. № 2. p. 151-155.Amariglio N., Rechavi G. A-to-I RNA editing: А new regulatory mechanism of global gene expression // Blood Cells Mol. Dis. 2007. V. 39. № 2. p. 151-155.Maas S., Kawahara Y., Tamburro K.M., Nishikura K. A-to-I RNA editing and human disease // RNA Biol. 2006. V. 3. № 1. p. 1-9.Maas S., Kawahara Y., Tamburro K.M., Nishikura K. A-to-I RNA editing and human disease // RNA Biol. 2006. V. 3. № 1. p. 1-9.Lopez-Saavedra A., Herrera L.A. The role of alternative mRNA splicing in chromosome instability // Mutat. Res. 2010. V. 705. № 3. p. 246-251.Lopez-Saavedra A., Herrera L.A. The role of alternative mRNA splicing in chromosome instability // Mutat. Res. 2010. V. 705. № 3. p. 246-251.Hallegger M., Llorian M., Smith C.W. Alternative splicing: Global insights // FEBS J. 2010. V. 277. № 4. p. 856-866.Hallegger M., Llorian M., Smith C.W. Alternative splicing: Global insights // FEBS J. 2010. V. 277. № 4. p. 856-866.David C. J., Manley J.L. Alternative pre-mRNA splicing regulation in cancer: Pathways and programs unhinged // Genes Dev. 2010. V. 24. № 21. p. 2343-2364.David C. J., Manley J.L. Alternative pre-mRNA splicing regulation in cancer: Pathways and programs unhinged // Genes Dev. 2010. V. 24. № 21. p. 2343-2364.Khanna A., Stamm S. Regulation of alternative splicing by short non-coding nuclear RNAs // RNA Biol. 2010. V. 7. № 4. p. 480-485.Khanna A., Stamm S. Regulation of alternative splicing by short non-coding nuclear RNAs // RNA Biol. 2010. V. 7. № 4. p. 480-485.Huang Y., Shen X.J., Zou Q., Zhao Q.L. Biological functions of microRNAs // Биоорган. химия. 2010. Т. 36. № 6. С. 747-752.Huang Y., Shen X.J., Zou Q., Zhao Q.L. Biological functions of microRNAs // Биоорган. химия. 2010. Т. 36. № 6. С. 747-752.Huntzinger E., Izaurralde E. Gene silencing by microRNAs: Сontributions of translational repression and mRNA decay // Nat. Rev. Genet. 2011. V. 12. № 2. p. 99-110.Huntzinger E., Izaurralde E. Gene silencing by microRNAs: Сontributions of translational repression and mRNA decay // Nat. Rev. Genet. 2011. V. 12. № 2. p. 99-110.Wang K.C., Chang H.Y. Molecular mechanisms of long noncoding RNAs // Mol. Cell. 2011. V. 43. № 6. p. 904-914.Wang K.C., Chang H.Y. Molecular mechanisms of long noncoding RNAs // Mol. Cell. 2011. V. 43. № 6. p. 904-914.Wapinski O., Chang H.Y. Long noncoding RNAs and human disease // Trends Cell Biol. 2011. V. 21. № 6. p. 354-361.Wapinski O., Chang H.Y. Long noncoding RNAs and human disease // Trends Cell Biol. 2011. V. 21. № 6. p. 354-361.Liao Q., Liu C., Yuan X., Kang S., Miao R., Xiao H., Zhao G., Luo H., Bu D., Zhao H., Skogerbо G., Wu Z., Zhao Y. Large-scale prediction of long non-coding RNA functions in a coding-non-coding gene co-expression network // Nucleic Acids Res. 2011. V. 39. № 9. p. 3864-3878.Liao Q., Liu C., Yuan X., Kang S., Miao R., Xiao H., Zhao G., Luo H., Bu D., Zhao H., Skogerbо G., Wu Z., Zhao Y. Large-scale prediction of long non-coding RNA functions in a coding-non-coding gene co-expression network // Nucleic Acids Res. 2011. V. 39. № 9. p. 3864-3878.Temin H.M. Proceedings: Molecular mechanisms for cancer induction by viruses // Proc. Natl. Cancer Conf. 1972. V. 7. P. 415-419.Temin H.M. Proceedings: Molecular mechanisms for cancer induction by viruses // Proc. Natl. Cancer Conf. 1972. V. 7. P. 415-419.Gilboa E., Mitra S. W., Goff S., Baltimore D. A detailed model of reverse transcription and tests of crucial aspects // Cell. 1979. V. 18. № 1. p. 93-100.Gilboa E., Mitra S. W., Goff S., Baltimore D. A detailed model of reverse transcription and tests of crucial aspects // Cell. 1979. V. 18. № 1. p. 93-100.Temin H.M. Retrovirus variation and reverse transcription: abnormal strand transfers result in retrovirus genetic variation // Proc. Natl. Acad. Sci. USA. 1993. V. 90. № 15. p. 6900-6903.Temin H.M. Retrovirus variation and reverse transcription: abnormal strand transfers result in retrovirus genetic variation // Proc. Natl. Acad. Sci. USA. 1993. V. 90. № 15. p. 6900-6903.Roy S. W., Irimia M. When good transcripts go bad: Artifactual RT-PCR 'splicing' and genome analysis // Bioessays. 2008. V. 30. № 6. p. 601-605.Roy S. W., Irimia M. When good transcripts go bad: Artifactual RT-PCR 'splicing' and genome analysis // Bioessays. 2008. V. 30. № 6. p. 601-605.Cocquet J., Chong A., Zhang G., Veitia R.A. Reverse transcriptase template switching and false alternative transcripts // Genomics. 2006. V. 88. № 1. p. 127-131.Cocquet J., Chong A., Zhang G., Veitia R.A. Reverse transcriptase template switching and false alternative transcripts // Genomics. 2006. V. 88. № 1. p. 127-131.Houseley J., Tollervey D. Apparent non-canonical trans-splicing is generated by reverse transcriptase in vitro // PLoS One. 2010. V. 5. № 8. p. e12271.Houseley J., Tollervey D. Apparent non-canonical trans-splicing is generated by reverse transcriptase in vitro // PLoS One. 2010. V. 5. № 8. p. e12271.International human genome consortium. Initial sequencing and analysis of the human genome // Nature. 2001. V. 409. № 6822. p. 860-921.International human genome consortium. Initial sequencing and analysis of the human genome // Nature. 2001. V. 409. № 6822. p. 860-921.Osenberg S, Dominissini D, Rechavi G., Eisenberg E. Widespread a cleavage of A-to-I hyperediting substrates // RNA. 2009. V. 15. № 9. P. 1632-1639.Osenberg S, Dominissini D, Rechavi G., Eisenberg E. Widespread a cleavage of A-to-I hyperediting substrates // RNA. 2009. V. 15. № 9. P. 1632-1639.Sambrook J., Russel D. Molecular cloning: A laboratory manual. - New York: Cold Spring Harbor Laboratory Press, 2001. 2344 p.Sambrook J., Russel D. Molecular cloning: A laboratory manual. - New York: Cold Spring Harbor Laboratory Press, 2001. 2344 p.
The authors declare that there are no conflicts of interest present.